Beta-galactosidase/GLB1 蛋白, Human (HEK293, His)

The beta-galactosidase/GLB1 protein cleaves beta-linked terminal galactose residues in gangliosides and glycoproteins, contributing to elastogenesis and connective tissue development. Beta-galactosidase/GLB1 Protein, Human (HEK293, His) is the recombinant human-derived Beta-galactosidase/GLB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Beta-galactosidase/GLB1 Protein, Human (HEK293, His) is 654 a.a., with molecular weight of ~90.0 kDa.

β-半乳糖苷酶 (GLB1) 蛋白作为一种酶，可从各种底物（包括神经节苷脂、糖蛋白和糖胺聚糖）中裂解 β 连接的末端半乳糖基残基。尽管缺乏直接的 β-半乳糖苷酶催化活性，GLB1 在弹性生成中发挥着重要的功能作用，有助于细胞外弹性纤维的形成和结缔组织的发育。有趣的是，GLB1 被确定为弹性蛋白结合蛋白 (EBP)，是成纤维细胞、平滑肌细胞、软骨细胞、白细胞和某些癌细胞类型上发现的非整联蛋白细胞表面受体的重要组成部分。在产生弹性蛋白的细胞中，GLB1 在细胞内与弹性蛋白原结合，作为回收分子伴侣，促进弹性蛋白原的分泌并支持其组装成弹性纤维。这种多方面的功能使 GLB1 在与组织发育和维护相关的酶促过程和结构作用中发挥关键作用。

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta-D-galactopyranoside. The specific activity is 1151.98 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

P16278 (L24-V677)

2720  [NCBI]

LRNATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

Approximately 90.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.