Beta-glucuronidase/GUSB 蛋白, E.coli (N-His,Solution)

The beta-glucuronidase/GUSB protein displays activity with PNPG and 4-methylumbelliferyl-glucuronide. It scavenges glucuronate from various xenobiotic and endobiotic glucuronides in the GI tract, utilizing diverse carbon sources. As part of the GI microbiome, it can reactivate glucuronide drug conjugates, potentially harming the GI tract. Beta-glucuronidase/GUSB Protein, E.coli (N-His,Solution) is the recombinant E. coli-derived Beta-glucuronidase/GUSB protein, expressed by E. coli , with N-6*His labeled tag. The total length of Beta-glucuronidase/GUSB Protein, E.coli (N-His,Solution) is 603 a.a., with molecular weight of 69-78 kDa.

β-葡萄糖醛酸酶/GUSB 蛋白具有 β-葡萄糖醛酸酶活性，通过人工底物对硝基苯基-β-D-葡萄糖醛酸苷 (PNPG) 和 4-甲基伞形酮基-葡萄糖醛酸苷证明。这种酶促能力表明，GUSB 可能擅长从胃肠道 (GI) 中存在的各种化学上不同的外源性和内生性葡萄糖醛酸中清除葡萄糖醛酸，从而利用不同的碳源。作为胃肠道微生物群的组成部分，GUSB 在葡萄糖醛酸药物缀合物的重新激活中发挥着至关重要的作用。然而，重新激活的化合物可能对胃肠道构成重大威胁，强调了 GUSB 对药物代谢的潜在影响以及胃肠道环境中微生物酶与宿主生理学之间复杂的相互作用。

Measured by its ability to hydrolyze Phenolphthalein Glucuronide. The specific activity is 1029.5 pmol/min/μg.

E.coli

E. coli

N-6*His

P05804 (M1-Q603)

946149  [NCBI]

MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

69-78 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.