1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Neurotrophic Factors
  4. Neurotrophins/NGF
  5. Nerve Growth Factor-β (Beta-NGF)
  6. Beta-NGF 蛋白, Human (HEK293)

Beta-NGF 蛋白, Human (HEK293)

目录号: HY-P7660
COA 产品使用指南

神经生长因子 (NGF) 是调节神经元存活和分化的神经营养因子,也是一种精浆蛋白,参与促排卵和促黄体作用。NGF 在女性生殖系统中具有潜在功能,有助于克服当前早期胚胎丢失的问题。 重组人 Beta-NGF 蛋白 (HEK293) ,是由 HEK293 细胞产生的重组蛋白 (S122-R239),不带标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥550 In-stock
50 μg ¥1800 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Nerve Growth Factor-β (Beta-NGF; NGF) is a neurotrophic factor that regulates neuronal survival and differentiation. NGF is also a seminal plasma protein involved in ovulation and luteinizing. NGF has potential functions in the female reproductive system to help overcome the current problem of early embryo loss[1][2][3]. Beta-NGF Protein, Human (HEK293) is produced by HEK293 cells (S122-R239), with tag free.

研究背景

神经生长因子-β(Beta-NGF;NGF)是一种由 118 个氨基酸组成的碱性蛋白,作为周围神经系统的感觉和交感神经元以及前基底大脑的胆碱能神经元的营养因子[1]。NGF 参与神经元存活和分化的调节。NGF 水平升高与创伤后应激障碍 (PTSD) 的风险相关。创伤反应导致负责 NGF 表达的 DNA 核苷酸甲基化。NGF 水平显示交感纤维密度的增加与 NGF 信使 RNA (mRNA) 水平成正比。NGF也是哺乳动物中常见的精浆蛋白。例如,NGF 作为骆驼的排卵刺激因子,并已被证明对公牛具有促黄体生成作用。NGF 在女性生殖系统中具有潜在的功能。例如,NGF在排卵诱导、LH释放、排卵、黄体发育、黄体酮(P4)产生、黄体体血管化和促性腺激素反应中发挥重要作用。对牛施用 NGF 可通过增加 LH 释放来增强类固醇产生、黄体形成和功能,并导致下游妊娠和发育标志物 mRNA 表达增加。此外,NGF 潜在的黄体生成作用有助于克服目前早期胚胎丢失的问题[2][3]。人和牛NGF蛋白序列相似度为90.87%。同时,人NGF与大鼠和小鼠的相似性分别为85.89%和85.06%。

体外研究
(In Vitro)

NGF (重组人源蛋白; 100 ng/mL; 5 d) 诱导 B 细胞的 DNA 合成呈剂量依赖性增加[4]

生物活性

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.04-0.4 ng/mL.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P01138 (S122-R239)

基因 ID
蛋白结构
N-term
Beta-NGF (S122-R239)
Accession # P01138
C-term
中文名
重组人 Beta-NGF 蛋白 (HEK293)
同用名
rHuBeta-NGF; Beta-Nerve Growth Factor; Beta-NGF;
氨基酸序列

SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVR

分子量

Approximately 14.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against 20 mM PB, 250 mM NaCl, pH7.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Beta-NGF Protein, Human (HEK293)
目录号:
HY-P7660
需求量: