1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. BirA 蛋白, E. coli (His-MBP)

BirA 蛋白, E. coli (His-MBP)

目录号: HY-P75475
COA 产品使用指南

所提供的段落中未提及 BirA 蛋白。如果您有其他段落或主题想要以 BirA 作为主题进行总结,请提供,我将很乐意为您提供帮助。BirA 蛋白, E. coli (His-MBP) 是重组的 BirA 蛋白,由 E. coli 表达,带有 N-His, N-MBP 标签。BirA 蛋白, E. coli (His-MBP) 全长 321 个氨基酸,分子量为 64-68 kDa。

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1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
50 μg ¥4700 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

BirA protein is not mentioned in the provided paragraph. If you have another paragraph or topic you'd like summarized with BirA as the subject, please provide it, and I'll be happy to assist. BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli, with N-His, N-MBP labeled tag. The total length of BirA Protein, E. coli (His-MBP) is 321 a.a., with molecular weight of 64-68 kDa.

研究背景

WBP2 蛋白在激素激活时充当雌激素和孕激素受体(ESR1 和 PGR)的转录共激活因子,在雌激素存在的情况下与 ESR1 响应启动子结合。它对于 YAP1 对 PGR 活性的共激活功能至关重要,并与 WBP2 协同增强 PGR 活性。此外,WBP2 通过调节 ESR1、ESR2 和 PGR 来调节突触后支架蛋白的表达。WBP2 与 YAP1、WWP1 和 WWP2 的 WW 结构域相互作用,还与 NEDD4 形成相互作用。此外,它与 ESR1 和 UBE3A 相关,有助于其在激素反应途径中作为转录共激活因子的作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

E.coli

表达系统

E. coli

标签

N-His;N-MBP

蛋白编号

P06709 (M1-K321)

基因 ID

948469  [NCBI]

蛋白结构
N-term
His-MBP
BirA (M1-K321)
Accession # P06709
C-term
同用名
Bifunctional ligase/repressor BirA; birA; bioR; dhbB
分子量

64-68 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

BirA Protein, E. coli (His-MBP) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BirA Protein, E. coli (His-MBP)
目录号:
HY-P75475
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