1. 重组蛋白
  2. Enzymes & Regulators
  3. birA 蛋白, E.coli (His)

birA 蛋白, E.coli (His)

目录号: HY-P702154
产品使用指南

birA蛋白具有生物素连接酶和生物素操纵子阻遏物的双重作用。在 ATP 存在的情况下,它会激活生物素形成 BirA-生物素基-5'-腺苷酸复合物。birA 蛋白, E.coli (His) 是重组的 birA 蛋白,由 E. coli 表达,带有 N-6*His 标签。birA 蛋白, E.coli (His) 全长 321 个氨基酸。

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描述

The birA protein has the dual role of biotin ligase and biotin operon repressor. In the presence of ATP, it activates biotin to form the BirA-biotinyl-5'-adenylate complex. birA Protein, E.coli (His) is the recombinant E. coli-derived birA protein, expressed by E. coli , with N-6*His labeled tag. The total length of birA Protein, E.coli (His) is 321 a.a., .

研究背景

BirA 蛋白具有生物素-[乙酰辅酶A-羧化酶] 连接酶和生物素-操纵子阻遏物的双重作用。在 ATP 存在的情况下,BirA 会激活生物素,从而形成 BirA-生物素基-5'-腺苷酸(BirA-bio-5'-AMP 或 holoBirA)复合物。HoloBirA 可以通过将生物素基转移到乙酰辅酶 A 羧化酶的生物素羧基载体蛋白 (BCCP) 亚基上来发挥作用,从而促进其活性。或者,holoBirA 可以与生物素操纵子位点结合并抑制操纵子的转录。尽管它被描述为孤儿受体,但 GPR146 蛋白的特定配体或功能仍然未知。

种属

E.coli

表达系统

E. coli

标签

N-6*His

蛋白编号

Q8X709 (M1-K321)

基因 ID

/

蛋白结构
N-term
6*His
birA (M1-K321)
Accession # Q8X709
C-term
同用名
birA; Bifunctional ligase/repressor BirA; Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] ligase; Biotin--protein ligase; Biotin-[acetyl-CoA carboxylase] synthetase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

birA Protein, E.coli (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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birA Protein, E.coli (His)
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