1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2) Protein Tyrosine Kinases
  4. B Lymphoid Tyrosine Kinase
  5. BLK 蛋白, Human (sf9, GST)

BLK 蛋白, Human (sf9, GST)

目录号: HY-P72853
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规格 是否有货
20 μg 询问价格 & 货期
50 μg 询问价格 & 货期

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  • 生物活性

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生物活性

The specific activity was determined to be 17.4 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

P51451 (M1-P505)

基因 ID

640  [NCBI]

同用名
Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk; BLK
氨基酸序列

MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP

分子量

Approximately 84 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE

性状

Solution.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 5 mM GSH, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BLK Protein, Human (sf9, GST)
目录号:
HY-P72853
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