1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. BLMH/Bleomycin 蛋白, Mouse (P. pastoris, His)

BLMH/Bleomycin 蛋白, Mouse (P. pastoris, His)

目录号: HY-P700626
产品使用指南

博莱霉素水解酶蛋白 (BLMH) 对于细胞防御至关重要,可通过水解其羧酰胺键来灭活抗肿瘤药物博莱霉素 (BLM)。这可以中和 BLM 的细胞毒性作用,保护正常和恶性细胞。BLMH 的确切生理功能尚不清楚,但其在维持细胞平衡和防御有害物质方面的重要性是显而易见的。BLMH/Bleomycin 蛋白, Mouse (P. pastoris, His) 是重组的 BLMH/博莱霉素蛋白,由 P. pastoris 表达,带有 N-6*His 标签。BLMH/Bleomycin 蛋白, Mouse (P. pastoris, His) 全长 455 个氨基酸,分子量为 53.8 kDa。

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描述

Bleomycin Hydrolase Protein (BLMH) is crucial for cellular defense, inactivating the antitumor drug Bleomycin (BLM) by hydrolyzing its carboxamide bond. This neutralizes BLM's cytotoxic effects, protecting normal and malignant cells. BLMH's exact physiological function is unknown, but its significance in maintaining cellular balance and defending against harmful agents is evident. BLMH/Bleomycin Protein, Mouse (P. pastoris, His) is the recombinant mouse-derived BLMH/Bleomycin protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of BLMH/Bleomycin Protein, Mouse (P. pastoris, His) is 455 a.a., with molecular weight of 53.8 kDa.

研究背景

BLM 水解酶,也称为博来霉素水解酶,通过催化抗肿瘤药物博来霉素 (BLM)(一种糖肽)的失活,在细胞防御机制中发挥着至关重要的作用。这种酶活性涉及 BLM 的 B-氨基丙氨酸酰胺部分内的羧酰胺键的水解,导致其失活。这个过程可以保护正常细胞和恶性细胞免受 BLM 的毒性作用。虽然 BLM 水解酶的精确生理功能尚不清楚,但其中和 BLM 细胞毒性影响的能力凸显了其在细胞稳态和防御潜在有害物质中的重要性。

种属

Mouse

表达系统

P. pastoris

标签

N-6*His

蛋白编号

Q8R016 (M1-E455)

基因 ID

104184  [NCBI]

蛋白结构
N-term
6*His
BLMH (M1-E455)
Accession # Q8R016
C-term
同用名
Bleomycin hydrolase; BH; BLM hydrolase; BMH
氨基酸序列

MNNAGLNSEKVSALIQKLNSDPQFVLAQNVGTTHDLLDICLRRATVQGAQHVFQHVVPQEGKPVTNQKSSGRCWIFSCLNVMRLPFMKKFNIEEFEFSQSYLFFWDKVERCYFFLNAFVDTAQKKEPEDGRLVQYLLMNPTNDGGQWDMLVNIVEKYGVVPKKCFPESHTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISSTQDAMMEEIFRVVCICLGNPPETFTWEYRDKDKNYHKIGPITPLQFYKEHVKPLFNMEDKICFVNDPRPQHKYNKLYTVDYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNGKLGLSDMNVYDHELVFGVSLKNMNKAERLAFGESLMTHAMTFTAVSEKDNQEGTFVKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDKKHVPEEVLAVLEQEPIVLPAWDPMGALAE

分子量

53.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BLMH/Bleomycin Protein, Mouse (P. pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BLMH/Bleomycin Protein, Mouse (P. pastoris, His)
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HY-P700626
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