1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Betacellulin
  5. BLOT2 蛋白, Blomia tropicalis (HEK293, His)

BLOT2 蛋白, Blomia tropicalis (HEK293, His)

目录号: HY-P77305
产品使用指南

BLOT1 蛋白是 Blomia tropicalis 过敏原,与半胱氨酸蛋白酶高度同源。BLOT1 可能作为一种免疫显性过敏原在尘螨特异性 IgE 介导的超敏反应中发挥重要作用。BLOT1 与其他 1 类 HDM 过敏原一样,是一种与螨虫粪便颗粒相关的消化酶。BLOT2 蛋白, Blomia tropicalis (HEK293, His) 是重组的 BLOT2 蛋白,由 HEK293 表达,带有 N-6*His 标签。BLOT2 蛋白, Blomia tropicalis (HEK293, His) 全长 144 个氨基酸,分子量约为 ~15 KDa。

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描述

BLOT1 Protein is a Blomia tropicalis allergen and is highly homologous to cysteine proteases. BLOT1 may play a major role as an immunodominant allergen involved in dust mite-specific IgE-mediated hypersensitivity. BLOT1, like the other group 1 HDM allergens, is a digestive enzyme associated with mite fecal pellets. BLOT2 Protein, Blomia tropicalis (HEK293, His) is the recombinant BLOT2 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of BLOT2 Protein, Blomia tropicalis (HEK293, His) is 144 a.a., with molecular weight of ~15 KDa.

研究背景

BLOT1 是 Blomia tropicalis 过敏原,与半胱氨酸蛋白酶高度同源。BLOT1 可能作为一种免疫显性过敏原在尘螨特异性 IgE 介导的超敏反应中发挥重要作用。BLOT1 与其他 1 类 HDM 过敏原一样,是一种与螨虫粪便颗粒相关的消化酶[1][2]

种属

Others

表达系统

HEK293

标签

N-6*His

蛋白编号

AAQ73483 (M1-D144)

基因 ID

/

蛋白结构
N-term
6*His
BLOT2 (M1-D144)
Accession # AAQ73483
C-term
同用名
Type 2 allergen Blo t 2.047
分子量

Approximately 15 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BLOT2 Protein, Blomia tropicalis (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BLOT2 Protein, Blomia tropicalis (HEK293, His)
目录号:
HY-P77305
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