1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Bone Morphogenetic Proteins (BMPs)
  5. Bone Morphogenetic Protein 2
  6. BMP-2 蛋白, Human/Mouse/Rat

BMP-2 蛋白, Human/Mouse/Rat

目录号: HY-P7006
COA 产品使用指南

骨形态发生蛋白2 (BMP-2) 是一种多效性配体蛋白,属于 TNFβ 家族,并参与关键胚胎发育的血管和瓣膜稳态。BMP-2 结合 I 型受体 (ALK-2/-3/-6) 和 II 型受体 (BMPR2, ACVR2A),以调节各种钙化类型,包括动脉粥样硬化、慢性肾病、糖尿病、瓣膜钙化。BMP-2 在人钙化瓣膜的骨化区域中由 B 淋巴细胞和 T 淋巴细胞密集浸润区域的肌成纤维细胞和前成骨细胞过度表达,是动脉粥样硬化血管钙化与正常骨形成机制之间的连接点。重组人/小鼠/大鼠 BMP-2 蛋白长 104 个氨基酸 (Q283-R396),由大肠杆菌表达。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥4600 In-stock
100 μg   询价  
250 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Bone morphogenetic protein 2 (BMP-2) is a pleiotropic ligand protein belonging to TNFβ family, and is involved in key embryonic development of vascular and valvular homeostasis. BMP-2 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A) to regulate various types of calcification, including atherosclerosis, chronic kidney disease, diabetes, and valve calcification[1]. BMP-2 is overexpressed by myofibroblast and preosteoblast in the calcified area of human calcified valve, which are densely infiltrated by B lymphocytes and T lymphocytes[2]. BMP-2 is the junction between atherosclerotic vascular calcification and normal bone formation mechanism[3]. BMP-2 Protein, Human/Mouse/Rat is 104 a.a. (Q283-R396), expressed in E. coli.

研究背景

Bone Morphogenetic Protein 2 (BMP-2) is a ligand protein with pleiotropic, belongs to TNFβ family. BMP-2 formats BMP/TGFβ signaling to involve in vascular and valvular homeostasis, which is a critical process of embryonic development[1].
BMP-2/TGFβ signaling can be terminated by inhibitory SMADs including SMAD6 and SMAD7, which are activated and induced by BMP signaling and switch off BMP signaling via multiple mechanisms[4].
BMP-2 is widely found in different animals, while the sequence in human is similar to Rat (91.86%), and mouse (92.13%).
BMPs exhibits critical contributions to the pathophysiology of atherosclerosis, pulmonary vascular disease, and vascular and valvular calcification[1].
BMP-2 binds different receptor, such as type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A), to regulate various calcification type including Atherosclerosis, Chronic Kidney Disease, Diabetes, Valvular Calcification[1].
BMP-2 promotes monocyte infiltration and inflammation of atherosclerotic legions[5].
It is linked to increased plaque formation via pro-inflammatory and pro-atherogenic effects, promoting oxidative stress, endothelial dysfunction and osteogenic differentiation[6].
BMP-2 is overexpressed in ossified regions of human calcified valves by myofibroblasts and pre-osteoblasts in areas densely infiltrated with B- and T-lymphocytes[2].
And it serves as the linkers between atherosclerotic vascular calcification with mechanisms of normal bone formation[3].
BMP-2 induces angiogenesis, endothelial cells (ECs) proliferation, and migration[7].
And BMP-2 also enhances the expression of the osteoblast and chondrocyte master transcriptional regulator RUNX2 to promote the mineralization of cultured human coronary vascular SMCs in a manner that was dependent on oxidative stress and endoplasmic reticulum (ER) stress[8].

体外研究
(In Vitro)

BMP-2 (10 nM; 15 min) shows little effect on the phosphorylation of MADR1, indicating MADR1 is a downstream component in the BMP2 signal transduction pathway[9].
BMP-2 (50 ng/mL; 14 d) induces osteogenic differentiation of mesenchymal stem cells (MSCs) as well as strong synergistic effect with 1 ng/mL VEGF and 10 ng/mL bFGF, in bone marrow of femurs and tibias of 6 week-old rats[10].

生物活性

Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells and the ED50 for this effect is <0.2 µg/ml.

种属

Human; Rat; Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P12643 (Q283-R396)

基因 ID

650  [NCBI]

中文名
重组骨形态发生蛋白 2
同用名
rHuBMP-2; BMP2A; BMP-2A; BMP2
氨基酸序列

MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

分子量

Approximately 26 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against 50 mM acetic acid.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 20 mM HAc or diluted with 5 mM HCl.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
BMP-2 Protein, Human/Mouse/Rat
目录号:
HY-P7006
需求量: