1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Bone Morphogenetic Proteins (BMPs)
  5. BMP-15
  6. BMP-15 蛋白, Human (His, Myc)

BMP-15 蛋白, Human (His, Myc)

目录号: HY-P72426
COA 产品使用指南

骨形态发生蛋白 15 (BMP-15;GDF9B) 又称生长分化因子 9B (GDF9B),是 TGFβ 家族的一种多效配体蛋白,仅在卵泡细胞中表达。BMP15 与 GDF9 密切相关,协同调控卵泡的遗传发育。BMP15 分别参与 p38 MAPK 和 HIF-1α/SCF 信号通路,可上调颗粒细胞抗苗勒氏激素 (AMH) 和多囊性卵巢综合征 (PCOS) 相关干细胞因子 (SCF) 的表达。人 BMP-15 蛋白全长 392 个氨基酸 (M1-R392),具有 4 个糖基化片段。重组人 BMP-15 蛋白 (His, Myc) 全长 125 个氨基酸 (Q268-R392),由 E. coli 大肠杆菌表达,在 N 端和 C 端分别带有 His 标签和 Myc 标签。

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描述

Bone morphogenetic protein 15 (BMP-15; GDF9B), also known as growth differentiation factor 9B (GDF9B), is a polymorphic ligand protein of the TGFβ family and is only expressed in follicular cells. BMP15 is closely related to GDF9 and synergistically regulates the genetic development of follicles. BMP15 is involved in p38 MAPK and HIF-1α/SCF signaling pathway, respectively, and can up-regulate the expression of anti-Mullerian hormone (AMH) and polycystic ovarian syndrome (PCOS) related stem cell factor (SCF) in granulosa cells. The total length of human BMP-15 protein is 392 amino acids (M1-R392), with 4 glycosylation domains. BMP-15 Protein, Human (His, Myc) has a total length of 125 amino acids (Q268-R392), is expressed in E. coli cells with a N-terminal His-tag, a C-terminal Myc-tag, respectively.

研究背景

骨形态发生蛋白 15(BMP-15;GDF9B)也称为生长和分化因子 9B (GDF9B),是一种属于 TGFβ 家族的多态性配体蛋白,仅在卵母细胞中表达[1] .
BMP15 与 GDF9 密切相关,GDF9 对于早期卵巢卵泡发生至关重要[1]
BMP15 和 GDF9 参与卵泡发育的遗传控制。其主要功能包括调节细胞增殖/分化、卵泡存活/闭锁和卵母细胞成熟,以创造支持卵泡选择和生长的环境[2]
BMP15 参与 p38 MAPK 通路上调颗粒细胞中抗苗勒管激素 (AMH) 的表达,该激素由窦前和小窦卵泡的颗粒细胞 (GC) 产生,在调节原始卵泡的募集和 FSH 依赖性卵泡发育中发挥作用< super>[3]。
另外,BMP15 结合 HIF-1α/SCF 信号通路诱导多囊卵巢综合征 (PCOS) 相关卵泡的人 GC 中干细胞因子 (SCF) 表达[4]
BMP-15广泛存在于不同的动物中,而人类的序列与大鼠(63.66%)和小鼠(64.01)不同。

体外研究
(In Vitro)

BMP-15 (huamn; 500 ng/mL; 48 h) induces FSHR expression in HGrC1 cells[5].
BMP-15 (huamn; 500 ng/mL; 0-90 min) increases phosphorylation of Smad 1/5/8 in HGrC1 cells[5].

种属

Human

表达系统

E. coli

标签

C-Myc;N-10*His

蛋白编号

O95972 (Q268-R392)

基因 ID
蛋白结构
N-term
10*His
BMP-15 (Q268-R392)
Accession # O95972
C-term
中文名
重组人 BMP-15 蛋白 (His, Myc)
同用名
Growth/differentiation factor 9B
氨基酸序列

QADGISAEVTASSSKHSGPENNQCSLHPFQISFRQLGWDHWIIAPPFYTPNYCKGTCLRVLRDGLNSPNHAIIQNLINQLVDQSVPRPSCVPYKYVPISVLMIEANGSILYKEYEGMIAESCTCR

分子量

Approximately 23.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BMP-15 Protein, Human (His, Myc)
目录号:
HY-P72426
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