BRCC36 蛋白, Human (His-SUMO)

BRCC36 is a metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains, especially histones H2A and H2AX in the BRCA1-A complex during the DNA damage response. As the catalytic subunit of the BRISC complex, it also targets “Lys-63”-linked ubiquitin in various substrates, affecting mitotic spindle assembly and interferon signaling. BRCC36 Protein, Human (His-SUMO) is the recombinant human-derived BRCC36 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of BRCC36 Protein, Human (His-SUMO) is 315 a.a., with molecular weight of ~51.9 kDa.

BRCC36 被鉴定为一种金属蛋白酶，在切割“Lys-63”连接的多聚泛蛋白链方面表现出特异性，而对“Lys-48”连接的多聚泛蛋白链没有活性。作为 BRCA1-A 复合物的重要组成部分，BRCC36 通过识别双链断裂位点的“Lys-63”连接的泛素化组蛋白 H2A 和 H2AX 来参与 DNA 损伤反应。在该复合体中，BRCC36 选择性去除组蛋白 H2A 和 H2AX 上的“Lys-63”连接泛素，对抗双链断裂处的 RNF8 依赖性泛素化。此外，BRCC36 还充当 BRISC 复合体的催化亚基，这是另一种多蛋白组装体，专门用于在各种底物中裂解“Lys-63”连接的泛素。BRISC 复合体参与有丝分裂纺锤体组装和微管附着于动粒，由 NUMA1 去泛素化介导，强调了其在细胞分裂中的调节作用。BRCC36 还通过干扰素受体 IFNAR1 的去泛素化来促进干扰素信号传导，从而增强其稳定性和细胞表面表达。此外，BRCC36通过介导NLRP3去泛素化而发挥调节NLRP3炎症小体的作用，导致炎症小体组装，并通过参与IFNAR1去泛素化下调对细菌脂多糖的反应。在其全部功能中，BRCC36 使 HDAC1 和 PWWP2B 去泛素化，从而有助于它们的稳定。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P46736 (A2-E316)

79184  [NCBI]

AVQVVQAVQAVHLESDAFLVCLNHALSTEKEEVMGLCIGELNDDTRSDSKFAYTGTEMRTVAEKVDAVRIVHIHSVIILRRSDKRKDRVEISPEQLSAASTEAERLAELTGRPMRVVGWYHSHPHITVWPSHVDVRTQAMYQMMDQGFVGLIFSCFIEDKNTKTGRVLYTCFQSIQAQKSSESLHGPRDFWSSSQHISIEGQKEEERYERIEIPIHIVPHVTIGKVCLESAVELPKILCQEEQDAYRRIHSLTHLDSVTKIHNGSVFTKNLCSQMSAVSGPLLQWLEDRLEQNQQHLQELQQEKEELMQELSSLE

Approximately 51.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of Tris-based buffer, 50% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.