1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. Bst DNA Polymerase 蛋白, Geobacillus stearothermophilus (His)

Bst DNA Polymerase 蛋白, Geobacillus stearothermophilus (His)

目录号: HY-P78942
产品使用指南

Bst DNA 聚合酶蛋白具有聚合酶和 5'-3' 核酸外切酶活性,具有多种核酸处理能力。除了合成 DNA 链之外,它还能熟练地从 5' 到 3' 方向去除核苷酸。这些双重功能使得 Bst DNA 聚合酶在分子生物学应用中发挥着关键作用,确保高效的 DNA 扩增和修饰过程。Bst DNA Polymerase 蛋白, Geobacillus stearothermophilus (His) 是重组的 Bst DNA 聚合酶蛋白,由 E. coli 表达,带有 N-6*His 标签。Bst DNA Polymerase 蛋白, Geobacillus stearothermophilus (His) 全长 584 个氨基酸,分子量约为 ~66.4 kDa。

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描述

Bst DNA Polymerase protein, featuring polymerase and 5'-3' exonuclease activities, exhibits versatile nucleic acid processing capabilities. Beyond synthesizing DNA strands, it adeptly removes nucleotides from the 5' to 3' direction. These dual functions render Bst DNA Polymerase pivotal in molecular biology applications, ensuring efficient DNA amplification and modification processes. Bst DNA Polymerase Protein, Geobacillus stearothermophilus (His) is the recombinant Bst DNA Polymerase protein, expressed by E. coli , with N-6*His labeled tag. The total length of Bst DNA Polymerase Protein, Geobacillus stearothermophilus (His) is 584 a.a., with molecular weight of ~66.4 kDa.

研究背景

除了聚合酶活性外,Bst DNA 聚合酶蛋白还具有 5'-3' 核酸外切酶活性。这种双重功能凸显了该酶在核酸加工中的多功能性,因为它不仅合成 DNA 链,还具有从 5' 到 3' 方向去除核苷酸的能力。聚合酶和核酸外切酶活性的联合作用使 Bst DNA 聚合酶成为各种分子生物学应用中的重要工具,有助于高效的 DNA 扩增和修饰过程。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

E1C9K5 (297-880)

基因 ID

/

蛋白结构
N-term
6*His
polA (297-880)
Accession # E1C9K5
C-term
氨基酸序列

DQSLFAIADSVTDEMLADKAALVVEVVGDNYHHAPIVGIALANERGRFFLRPETALADPKFLAWLGDETKKKTMFDSKRAAVALKWKGIELRGVVFDLLLAAYLLDPAQAAGDVAAVAKMHQYEAVRSDEAVYGKGAKRTVPDEPTLAEHLVRKAAAIWALEEPLMDELRRNEQDRLLTELEQPLAGILANMEFTGVKVDTKRLEQMGAELTEQLQAVERRIYELAGQEFNINSPKQLGTVLFDKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVEHILHYRQLGKLQSTYIEGLLKVVHPVTGKVHTMFNQALTQTGRLSSVEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIELRVLAHIAEDDNLIEAFRRGLDIHTKTAMDIFHVSEEDVTANMRRQAKAVNFGIVYGISDYGLAQNLNITRKEAAEFIERYFASFPGVKQYMDNIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLSVRLREERLQARLLLQVHDELILEAPKEEIERLCRLVPEVMEQAVTLRVPLKVDYHYGPTWYDAK

分子量

Approximately 66.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

文件资料

Bst DNA Polymerase Protein, Geobacillus stearothermophilus (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Bst DNA Polymerase Protein, Geobacillus stearothermophilus (His)
目录号:
HY-P78942
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