BST2 蛋白, Mouse (HEK293, His)

The BST2 protein is an IFN-induced antiviral factor that inhibits mammalian enveloped viruses by tethering viral particles to infected cell membranes. BST2 acts as a physical tether connecting virions, limiting their release and spread. BST2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived BST2 protein, expressed by HEK293 , with N-His labeled tag. The total length of BST2 Protein, Mouse (HEK293, His) is 100 a.a., with molecular weight of ~13.6-24 kDa.

BST2 蛋白是一种 IFN 诱导的抗病毒宿主限制因子，通过将新生病毒颗粒直接束缚在受感染细胞的膜上，可作为多种哺乳动物包膜病毒的有效抑制剂。BST2 作为直接的物理系链，将病毒体固定在细胞膜上，促进它们相互连接。这种独特的机制抑制病毒颗粒的释放，病毒颗粒可以通过内吞作用内化并随后降解，或者保留在细胞表面，限制它们作为无细胞病毒颗粒的传播。BST2 针对不同科的病毒，包括逆转录病毒科（例如，HIV-1、MMTV、MLV）、丝状病毒科（例如，EBOV）、沙粒病毒科（例如，LASV）和弹状病毒科（例如，VSV），表现出广泛的抗病毒活性。除了在病毒限制中的作用外，BST2 还抑制 MMP14 的细胞表面蛋白水解活性，导致 MMP15 的激活减少，从而抑制细胞生长和迁移。此外，BST2 可以刺激 LILRA4/ILT7 的信号传导，为浆细胞样树突状细胞响应病毒感染而产生 IFN 提供负反馈。此外，BST2 有助于极化上皮细胞中顶端下肌动蛋白细胞骨架的组织。在结构上，BST2 形成二硫键连接的平行同源二聚体，其二聚化对其抗病毒活性至关重要。该蛋白与 ARHGAP44、MMP14 和 LILRA4/ILT7 相互作用，揭示其参与多种细胞过程和信号传导途径。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse BST-2/Tetherin is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Viral Dengue Virus 4 Envelope. The ED₅₀ for this effect is 0.349 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse BST-2/Tetherin is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Viral Dengue Virus 4 Envelope.The ED₅₀ for this effect is 0.349 μg/mL.

Mouse

HEK293

N-His

Q8R2Q8 (T52-N151)

69550  [NCBI]

TANSVACRDGLRAQAECRNTTHLLQRQLTRTQDSLLQAETQANSCNLTVVTLQESLEKKVSQALEQQARIKELENEVTKLNQELENLRIQKETSSTVQVN

Approximately 13.6-24 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.