BTK 蛋白, Human (P. pastoris, His)

BTK Protein, a key non-receptor tyrosine kinase, is essential for B lymphocyte development and signaling. Upon BCR activation, BTK initiates a cascade, phosphorylating PLCG2 and activating downstream pathways, influencing calcium mobilization and PKC activation. In innate and adaptive immunity, particularly TLR pathways, BTK regulates TLR9 activation, induces NF-kappa-B activity, and activates NLRP3 inflammasome assembly. Its multifaceted functions include phosphorylating GTF2I, participating in apoptosis regulation, and serving as a platform for diverse signaling proteins. BTK Protein, Human (P. pastoris, His) is the recombinant human-derived BTK protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of BTK Protein, Human (P. pastoris, His) is 659 a.a., with molecular weight of 78.2 kDa.

BTK 蛋白是一种非受体酪氨酸激酶，对于 B 淋巴细胞的发育、分化和信号传导是不可或缺的。抗原与 B 细胞抗原受体 (BCR) 结合后，BTK 会触发信号级联，导致 B 细胞激活、磷酸化 PLCG2 并启动涉及钙动员和蛋白激酶 C (PKC) 激活的下游途径。BTK 作为多种信号蛋白的平台，参与细胞因子受体信号通路，在先天性和适应性免疫中至关重要，特别是在 Toll 样受体 (TLR) 通路中。它在调节脾 B 细胞中的 TLR9 激活中发挥着关键作用，并诱导 NF-kappa-B 的活性，从而有助于众多基因的转录调节。此外，BTK 还能激活 NLRP3 炎症小体组装、磷酸化转录因子 GTF2I，并参与细胞凋亡调节，凸显其在免疫反应和细胞过程中的多方面功能。

Human

P. pastoris

N-6*His

Q06187 (M1-S659)

695  [NCBI]

AAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

78.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.