C-Reactive 蛋白, Rat (HEK293, His)

Reactive protein (CRP) performs multiple functions of host defense, including promoting agglutination, bacterial capsule swelling, phagocytosis, and complement fixation. These activities are promoted by its calcium-dependent binding to phosphocholine, suggesting its role in orchestrating immune responses against pathogens. C-Reactive Protein, Rat (HEK293, His) is the recombinant rat-derived C-Reactive protein, expressed by HEK293 , with C-His labeled tag. The total length of C-Reactive Protein, Rat (HEK293, His) is 211 a.a., with molecular weight of ~30 kDa.

反应蛋白 (CRP) 在宿主防御中发挥多方面的作用，展示了免疫反应所必需的多种功能。CRP 通过以钙依赖性方式与磷酸胆碱结合，促进凝集、细菌荚膜肿胀、吞噬作用和补体固定。此外，CRP 表现出与 DNA 和组蛋白相互作用的能力，表明其在清除受损循环细胞释放的核物质方面具有潜在作用。这些不同的功能强调了 CRP 参与免疫系统的各个方面，有助于先天性和适应性免疫反应，并强调其作为宿主防御机制中关键参与者的重要性（参考文献不可用）。

Immobilized Recombinant C-Reactive Protein/CRP at 1 μg/ml (100 μL/well) can bind Biotinylated Recombinant Human Fc gamma RIIA/CD32a Protein. The ED₅₀ for this effect is 1.821 μg/mL.

Rat

HEK293

C-His

P48199 (H20-S230)

25419  [NCBI]

HEDMSKQAFVFPGVSATAYVSLEAESKKPLEAFTVCLYAHADVSRSFSIFSYATKTSFNEILLFWTRGQGFSIAVGGPEILFSASEIPEVPTHICATWESATGIVELWLDGKPRVRKSLQKGYIVGTNASIILGQEQDSYGGGFDANQSLVGDIGDVNMWDFVLSPEQINAVYVGRVFSPNVLNWRALKYETHGDVFIKPQLWPLTDCCES

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.