1. 重组蛋白
  2. Complement System
  3. Complement Component 2
  4. C2/Complement C2 蛋白, Human (HEK293, His)

C2/Complement C2 蛋白, Human (HEK293, His)

目录号: HY-P74375
COA 产品使用指南

C2/补体 C2 蛋白在补体系统的经典途径中至关重要。被因子 C1 激活后,C2 裂解为 C2b 和 C2a。作为丝氨酸蛋白酶,C2a 与 C4b 相互作用,产生 C3 或 C5 转化酶。这一关键过程激活并放大补体级联。C2/Complement C2 蛋白, Human (HEK293, His) 是重组的 C2/补体 C2 蛋白,由 HEK293 表达,带有 C-His 标签。C2/Complement C2 蛋白, Human (HEK293, His) 全长 732 个氨基酸,分子量约为 ~112 kDa。

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描述

C2/Complement C2 protein is essential in the classical pathway of the complement system. Activated by factor C1, C2 is cleaved into C2b and C2a. As a serine protease, C2a interacts with C4b, generating the C3 or C5 convertase. This crucial process activates and amplifies the complement cascade. C2/Complement C2 Protein, Human (HEK293, His) is the recombinant human-derived C2/Complement C2 protein, expressed by HEK293 , with C-His labeled tag. The total length of C2/Complement C2 Protein, Human (HEK293, His) is 732 a.a., with molecular weight of ~112 kDa.

研究背景

C2/补体 C2 蛋白在补体系统的经典途径中起着至关重要的作用。当被因子 C1 激活时,C2 发生裂解,形成两个片段:C2b 和 C2a。C2a 作为丝氨酸蛋白酶起作用,随后与补体因子 C4b 相互作用,导致 C3 或 C5 转化酶的产生。这个过程对于补体级联的激活和放大至关重要。

生物活性

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 165.13 pmol/min/μg.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P06681 (A21-L752)

基因 ID

717  [NCBI]

蛋白结构
N-term
C2 (A21-L752)
Accession # P06681
His
C-term
中文名
重组人C2蛋白
同用名
Complement C2; C3/C5 convertase; C2
氨基酸序列

APSCPQNVNISGGTFTLSHGWAPGSLLTYSCPQGLYPSPASRLCKSSGQWQTPGATRSLSKAVCKPVRCPAPVSFENGIYTPRLGSYPVGGNVSFECEDGFILRGSPVRQCRPNGMWDGETAVCDNGAGHCPNPGISLGAVRTGFRFGHGDKVRYRCSSNLVLTGSSERECQGNGVWSGTEPICRQPYSYDFPEDVAPALGTSFSHMLGATNPTQKTKESLGRKIQIQRSGHLNLYLLLDCSQSVSENDFLIFKESASLMVDRIFSFEINVSVAIITFASEPKVLMSVLNDNSRDMTEVISSLENANYKDHENGTGTNTYAALNSVYLMMNNQMRLLGMETMAWQEIRHAIILLTDGKSNMGGSPKTAVDHIREILNINQKRNDYLDIYAIGVGKLDVDWRELNELGSKKDGERHAFILQDTKALHQVFEHMLDVSKLTDTICGVGNMSANASDQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRDGNDHSLWRVNVGDPKSQWGKEFLIEKAVISPGFDVFAKKNQGILEFYGDDIALLKLAQKVKMSTHARPICLPCTMEANLALRRPQGSTCRDHENELLNKQSVPAHFVALNGSKLNINLKMGVEWTSCAEVVSQEKTMFPNLTDVREVVTDQFLCSGTQEDESPCKGESGGAVFLERRFRFFQVGLVSWGLYNPCLGSADKNSRKRAPRSKVPPPRDFHINLFRMQPWLRQHLGDVLNFLPL

分子量

Approximately 112 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

C2/Complement C2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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