CADM1/IGSF4A 蛋白, Human (HEK293, His)

The CADM1/IGSF4A protein is an important mediator of cell-cell adhesion. It forms homodimers and interacts with CADM3 and NECTIN3. CADM1/IGSF4A Protein, Human (HEK293, His) is the recombinant human-derived CADM1/IGSF4A protein, expressed by HEK293 , with C-His labeled tag. The total length of CADM1/IGSF4A Protein, Human (HEK293, His) is 330 a.a., with molecular weight of 60-95 kDa.

CADM1/IGSF4A 蛋白作为嗜同性和异嗜细胞-细胞粘附的关键介质，以不依赖 Ca(2+) 的方式起作用。它形成同型二聚体并介导同型粘附，并且还参与与 CADM3 和 NECTIN3 的异型相互作用。CADM1 和 CRTAM 之间的相互作用可促进自然杀伤 (NK) 细胞的细胞毒性、CD8+ 细胞分泌干扰素-γ (IFN-γ)，以及 NK 细胞介导的表达 CADM1 的肿瘤的排斥反应。在肥大细胞中，CADM1 在神经的附着和通讯中发挥着至关重要的作用，对于肥大细胞在体内的发育和存活至关重要。此外，CADM1 参与引流淋巴结内 T 细胞滞留的调节，这是特定 T 细胞亚群肠道滞留所必需的，并有助于 T 细胞与肠道相关树突状细胞的粘附。此外，CADM1 还充当突触细胞粘附分子，在树突棘形成和突触组装中发挥作用。它涉及神经元过程，例如迁移、轴突生长、寻路和束颤。此外，CADM1 参与精子发生，有助于精母细胞和精子细胞与支持细胞的粘附。在非小细胞肺癌 (NSCLC) 细胞中，CADM1 充当肿瘤抑制因子，影响侵袭表型。它通过其 Ig 样 V 型结构域形成同二聚体，并与各种蛋白质相互作用，包括 FARP1、CRTAM、EPB41L3/DAL1、MPP2、MPP3 和 PALS2，促进多种细胞功能和信号通路。

Measured by its binding ability in a functional ELISA. Immobilized Human CRTAM at 2 μg/mL (100 μL/well) can bind Biotinylated Human CADM1. The ED₅₀ for this effect is 105.7 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human CRTAM at 2 μg/mL (100 μL/well) can bind Biotinylated Human CADM1. The ED₅₀ for this effect is 105.7 ng/mL.

Human

HEK293

C-His

Q9BY67 (Q45-H374)

23705  [NCBI]

QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDH

60-95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.