Calmodulin 蛋白, Human

Calmodulin Protein, Human is a recombinant human Calmodulin expressed in E. coli. Calmodulin is a low molecular weight, acidic, calcium binding protein which mediates the Ca²⁺ regulation of a wide range of physiological processes throughout eukaryotic organisms^[1].

在低游离 Ca²⁺ 浓度下，例如存在于静息肌肉肌浆中，钙调蛋白以无 Ca²⁺ 形式存在，在这种状态下，它通常不与目标蛋白。在适当的刺激后，游离 Ca²⁺ 浓度升高，Ca²⁺ 与钙调蛋白结合，钙调蛋白发生构象变化，使其能够与目标蛋白相互作用^[1]。

206875 units per mg protein. One unit is defined as the amount of calmodulin which will give rise to 50% of the maximal enzyme activation of a standard level of activator-deficient calcineurin.

Human

E. coli

Tag Free

P0DP23 (M1-K149)

801  [NCBI]/ 805  [NCBI]/ 808  [NCBI]

MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK

Approximately 16.0-17.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 50 mM NH₄HCO₃, pH 8.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. M P Walsh, et al. Calmodulin and its roles in skeletal muscle function. Can Anaesth Soc J. 1983 Jul;30(4):390-8.  [Content Brief]