2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. CAMKI gamma/CAMK1G 蛋白, Human (sf9, His, GST)

CAMKI gamma/CAMK1G 蛋白, Human (sf9, His, GST)

目录号: HY-P76191
产品使用指南

CAMK1G 蛋白是一种钙/钙调蛋白依赖性蛋白激酶，也是所提出的钙触发信号级联的关键组成部分。该激酶表现出体外磷酸化转录因子 CREB1 的能力，表明其参与调节与基因表达和转录控制相关的细胞过程。CAMKI gamma/CAMK1G 蛋白, Human (sf9, His-GST) 是重组的 CAMKI gamma/CAMK1G 蛋白，由 Sf9 insect cells 表达，带有 N-His, N-GST 标签。CAMKI gamma/CAMK1G 蛋白, Human (sf9, His-GST) 全长 476 个氨基酸，分子量约为 ~75 KDa。

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3.  试用装只面向终端客户。

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描述

The CAMK1G protein is a calcium/calmodulin-dependent protein kinase and a key component of the proposed calcium-triggered signaling cascade. This kinase exhibits the ability to phosphorylate the transcription factor CREB1 in vitro, suggesting its involvement in regulating cellular processes related to gene expression and transcriptional control. CAMKI gamma/CAMK1G Protein, Human (sf9, His, GST) is the recombinant human-derived CAMKI gamma/CAMK1G protein, expressed by Sf9 insect cells , with His, GST labeled tag. The total length of CAMKI gamma/CAMK1G Protein, Human (sf9, His, GST) is 476 a.a., with molecular weight of ~75 KDa.
研究背景

CAMK1G，也称为钙/钙调蛋白依赖性蛋白激酶 I gamma，是 CAMK 家族的重要成员，参与拟议的钙触发信号级联。该蛋白具有激酶活性，特别是通过其转录因子 CREB1（cAMP 反应元件结合蛋白 1）的磷酸化在体外得到证明，表明其在调节基因表达中发挥作用。CAMK1G 的激活取决于钙和钙调蛋白，强调其参与响应钙水平变化的细胞内信号传导途径。CAMK1G 在通过磷酸化转导钙信号和调节下游细胞事件方面具有潜在意义，有助于更广泛地了解其在细胞过程中的功能作用。
种属

Human
表达系统

Sf9 insect cells
标签

His;GST
蛋白编号

Q96NX5-1 (M1-M476)
基因 ID
同用名
Calcium/calmodulin-dependent protein kinase type 1G; CaMKI gamma; CLICK III; CLICK3; VWS1
分子量

Approximately 75 kDa.
纯度

Greater than 85% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CAMKI gamma/CAMK1G Protein, Human (sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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