2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. CAPN1 蛋白, Mouse (His)

CAPN1 蛋白, Mouse (His)

目录号: HY-P72114
产品使用指南

CAPN1 蛋白是一种钙调节的非溶酶体硫醇蛋白酶，促进细胞骨架重塑和信号转导。它选择性地切割参与这些过程的底物，包括“Asn-375”、“Gly-388”和“His-410”处的 CTBP1。CAPN1 还激活 caspase-7 (CASP7)，突出其在调节细胞功能中的作用。CAPN1 蛋白, Mouse (His) 是重组的 CAPN1 蛋白，由 E. coli 表达，带有 N-6*His 标签。CAPN1 蛋白, Mouse (His) 全长 713 个氨基酸，分子量约为 ~86.1 kDa。

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描述

CAPN1 protein, a calcium-regulated non-lysosomal thiol-protease, facilitates cytoskeletal remodeling and signal transduction. It selectively cleaves substrates involved in these processes, including CTBP1 at 'Asn-375', 'Gly-388', and 'His-410'. CAPN1 also activates caspase-7 (CASP7), highlighting its role in regulating cellular functions. CAPN1 Protein, Mouse (His) is the recombinant mouse-derived CAPN1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CAPN1 Protein, Mouse (His) is 713 a.a., with molecular weight of ~86.1 kDa.
研究背景

CAPN1 是一种钙调节的非溶酶体硫醇蛋白酶，在细胞骨架重塑和信号转导中发挥着至关重要的作用。它选择性地催化这些过程中涉及的底物的有限蛋白水解。具体来说，CAPN1 靶向 CTBP1 并在“Asn-375”、“Gly-388”和“His-410”处切割它。此外，CAPN1 负责 caspase-7 (CASP7) 的裂解和激活，进一步强调了其在调节细胞功能中的重要性。
种属

Mouse
表达系统

E. coli
标签

N-6*His
蛋白编号

O35350 (M1-A713)
基因 ID

12333  [NCBI]
蛋白结构
N-term
6*His
CAPN1 (M1-A713)
Accession # O35350
C-term
同用名
Capn1; Canp1; Capa1Calpain-1 catalytic subunit; EC 3.4.22.52; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Micromolar-calpain; muCANP
氨基酸序列

MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVTFDLFKWLQLTMFA

分子量

Approximately 86.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CAPN1 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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