2. 重组蛋白
  3. Enzymes & Regulators
  4. Lyases (EC 4) Carbonic Anhydrase
  5. Carbonic Anhydrase 1 (CA1)
  6. Carbonic Anhydrase 1 蛋白, Human (His)

Carbonic Anhydrase 1 蛋白, Human (His)

目录号: HY-P7718
COA 产品使用指南

Carbonic Anhydrase 1 蛋白, Human (His) 在 E. coli 中表达，N 端带有一个 His 标签。Carbonic Anhydrase 1 表达和 CA1 介导的钙化与动脉粥样硬化 (AS) 进展被显著相关联。

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规格 价格 是否有货 数量
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2 μg ￥350 In-stock
10 μg ￥860 In-stock
50 μg ￥2400 In-stock
100 μg   询价  

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描述

Carbonic Anhydrase 1 Protein, Human (His) expresses in E. coli with a His tag at the N-terminus. Carbonic Anhydrase 1 (CA1) expression and CA1-mediated calcification are significantly associated with atherosclerosis (AS) progression[1].
研究背景

碳酸酐酶 1 (CA1) 是碳酸酐酶 (CA) 家族的成员，可可逆地催化 CO2 水合形成 HCO3-，然后迅速与钙离子结合形成碳酸钙。CA1 在人类 AS 组织和 β-磷酸甘油诱导钙化的大鼠血管平滑肌细胞 (VSMC) 中也高表达[1]。
生物活性

The esterase activity is determined to be greater than 500 pmol/min/μg.
种属

Human
表达系统

E. coli
标签

C-6*His
蛋白编号

P00915 (S2-F261)
基因 ID

759  [NCBI]
蛋白结构
N-term
CA1 (S2-F261)
Accession # P00915
6*His
C-term
中文名
重组人碳酸酐酶 1, His
同用名
rHuCarbonic Anhydrase 1, His; Carbonic Anhydrase 1; Carbonate Dehydratase I; Carbonic Anhydrase B; CAB; Carbonic Anhydrase I; CA1
氨基酸序列

ASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASFHHHHHH

分子量

25-35 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE. 
性状

溶液
组分

Supplied as a 0.2 μm filter solution of 12.5 mM Tris-HCl, 75 mM NaCl, pH 7.5.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)
参考文献

Carbonic Anhydrase 1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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