1. 重组蛋白
  2. Enzymes & Regulators
  3. Carbonic Anhydrase
  4. Carbonic Anhydrase 10
  5. Carbonic Anhydrase 10 蛋白, Human (His)

Carbonic Anhydrase 10 蛋白, Human (His)

目录号: HY-P7725
产品使用指南

Carbonic Anhydrase 10 蛋白, Human (His)在 E. coli 中表达,N 端带有一个 His 标签。Carbonic anhydrase 10 是碳酸酐酶 (CAs) 的一种无催化活性的亚型。

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描述

Carbonic Anhydrase 10 Protein, Human (His) expresses in E. coli with a His tag at the N-terminus. Carbonic anhydrase 10 is an inactive isoform of carbonic anhydrase (CAs)[1].

研究背景

碳酸酐酶 (CA) 的无催化活性亚型被称为 CA 相关蛋白 (CARP) VIII、X 和 XI。它们具有高度保守的氨基酸序列。与白天相比,CARP X 在夜间在松果体中表达更高,表明其对唤醒/睡眠模式有一定的作用[1]

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

Q9NS85 (A21-N300)

基因 ID
蛋白结构
N-term
CA10 (A21-N300)
Accession # Q9NS85
6*His
C-term
中文名
重组人碳酸酐酶 10, His
同用名
rHuCarbonic Anhydrase 10, His; Carbonic Anhydrase-Related Protein 10; Carbonic Anhydrase-Related Protein X; CARP X; Cerebral Protein 15; CA10;
氨基酸序列

AQQNSPKIHEGWWAYKEVVQGSFVPVPSFWGLVNSAWNLCSVGKRQSPVNIETSHMIFDPFLTPLRINTGGRKVSGTMYNTGRHVSLRLDKEHLVNISGGPMTYSHRLEEIRLHFGSEDSQGSEHLLNGQAFSGEVQLIHYNHELYTNVTEAAKSPNGLVVVSIFIKVSDSSNPFLNRMLNRDTITRITYKNDAYLLQGLNIEELYPETSSFITYDGSMTIPPCYETASWIIMNKPVYITRMQMHSLRLLSQNQPSQIFLSMSDNFRPVQPLNNRCIRTNHHHHHH

分子量

Approximately 31.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

Carbonic Anhydrase 10 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Carbonic Anhydrase 10 Protein, Human (His)
目录号:
HY-P7725
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