2. 重组蛋白
  3. Enzymes & Regulators
  4. Lyases (EC 4) Carbonic Anhydrase
  5. Carbonic Anhydrase 14 (CA-XIV)
  6. Carbonic Anhydrase 14 蛋白, Human (HEK293, His)

Carbonic Anhydrase 14 蛋白, Human (HEK293, His)

目录号: HY-P72867
产品使用指南

碳酸酐酶 14 蛋白催化二氧化碳的可逆水合，将其转化为碳酸氢根离子和质子，反之亦然。其重要的酶活性有助于生理过程、调节酸碱平衡、呼吸和细胞 pH 稳态。该蛋白质在体内二氧化碳运输和新陈代谢中发挥着关键作用。Carbonic Anhydrase 14 蛋白, Human (HEK293, His) 是重组的 碳酸酐酶 14 蛋白，由 HEK293 表达，带有 C-His 标签。Carbonic Anhydrase 14 蛋白, Human (HEK293, His) 全长 275 个氨基酸，分子量为 45-48 kDa。

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规格 是否有货
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100 μg   询价  

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描述

Carbonic Anhydrase 14 Protein catalyzes the reversible hydration of carbon dioxide, converting it to bicarbonate ions and protons, and vice versa. Its vital enzymatic activity contributes to physiological processes, regulating acid-base balance, respiration, and cellular pH homeostasis. The protein plays a pivotal role in carbon dioxide transport and metabolism in the body. Carbonic Anhydrase 14 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 14 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carbonic Anhydrase 14 Protein, Human (HEK293, His) is 275 a.a., with molecular weight of 45-48 kDa.
研究背景

碳酸酐酶 14 蛋白是一种催化二氧化碳可逆水合的酶。其主要功能是促进二氧化碳转化为碳酸氢根离子和质子，反之亦然。这种酶活性在各种生理过程中至关重要，包括酸碱平衡调节、呼吸和细胞 pH 稳态的维持。碳酸酐酶 14 蛋白在体内二氧化碳运输和代谢中发挥着关键作用。
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

Q9ULX7/NP_036245.1 (A16-M290)
基因 ID
蛋白结构
N-term
CA14 (A16-M290)
Accession # Q9ULX7/NP_036245.1
His
C-term
同用名
Carbonic anhydrase 14; Carbonate dehydratase XIV; CA-XIV; CA14
氨基酸序列

MLFSALLLEVIWILAADGGQHWTYEGPHGQDHWPASYPECGNNAQSPIDIQTDSVTFDPDLPALQPHGYDQPGTEPLDLHNNGHTVQLSLPSTLYLGGLPRKYVAAQLHLHWGQKGSPGGSEHQINSEATFAELHIVHYDSDSYDSLSEAAERPQGLAVLGILIEVGETKNIAYEHILSHLHEVRHKDQKTSVPPFNLRELLPKQLGQYFRYNGSLTTPPCYQSVLWTVFYRRSQISMEQLEKLQGTLFSTEEEPSKLLVQNYRALQPLNQRMVFASFIQAGSSYTTGEM

分子量

45-48 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Carbonic Anhydrase 14 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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