1. 重组蛋白
  2. CAR-T related Proteins Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 9 (CA IX)
  5. Carbonic Anhydrase 9 蛋白, Cynomolgus (HEK293, His)

Carbonic Anhydrase 9 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P700882
COA 产品使用指南

碳酸酐酶 9 蛋白催化二氧化碳的可逆水合,这对于将其转化为碳酸氢根离子和质子至关重要,反之亦然。这种酶活性对于酸碱平衡调节、呼吸和细胞 pH 稳态等生理过程至关重要。碳酸酐酶 9 蛋白对于二氧化碳运输和代谢至关重要,有助于对其在体内水平的整体调节。Carbonic Anhydrase 9 蛋白, Cynomolgus (HEK293, His) 是重组的 碳酸酐酶 9 蛋白,由 HEK293 表达,带有 C-His 标签。Carbonic Anhydrase 9 蛋白, Cynomolgus (HEK293, His) 全长 365 个氨基酸,分子量为 50-60 kDa。

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规格 价格 是否有货 数量
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5 μg ¥740 In-stock
10 μg ¥1250 In-stock
50 μg ¥3500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Carbonic Anhydrase 9 Protein catalyzes the reversible hydration of carbon dioxide, essential for converting it to bicarbonate ions and protons, and vice versa. This enzymatic activity is crucial in physiological processes like acid-base balance regulation, respiration, and cellular pH homeostasis. Carbonic Anhydrase 9 Protein is vital for carbon dioxide transport and metabolism, contributing to overall regulation of its levels in the body. Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is 365 a.a., with molecular weight of 50-60 kDa.

研究背景

碳酸酐酶 9 蛋白是一种促进二氧化碳可逆水合的酶。它在将二氧化碳转化为碳酸氢根离子和质子及其逆反应中起着至关重要的作用。这种酶活性对于酸碱平衡调节、呼吸和细胞 pH 稳态维持等各种生理过程至关重要。碳酸酐酶 9 蛋白对于体内二氧化碳的运输和代谢至关重要,有助于二氧化碳水平的整体调节。

生物活性

Immobilized Cynomolgus CA9, His Tag at 0.5 μg/mL (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of <10.1 ng/mL determined by ELISA.

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

A0A2K5VQG9 (Q38-D402)

基因 ID

/

蛋白结构
N-term
CA9 (Q38-D402)
Accession # A0A2K5VQG9
His
C-term
同用名
CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
分子量

50-60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P700882
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