2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Carboxyl Ester Lipase/CEL 蛋白, Mouse (sf9, His)

Carboxyl Ester Lipase/CEL 蛋白, Mouse (sf9, His)

目录号: HY-P74352
产品使用指南

羧酸酯脂肪酶/CEL 蛋白在细胞过程中起着至关重要的作用，因为它催化多种底物的水解，包括胆固醇酯、磷脂、溶血磷脂、二酰基甘油和三酰基甘油以及羟基脂肪酸酯的脂质。FAHF）。Carboxyl Ester Lipase/CEL 蛋白, Mouse (sf9, His) 是重组的 羧基酯脂肪酶/CEL 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。Carboxyl Ester Lipase/CEL 蛋白, Mouse (sf9, His) 全长 534 个氨基酸，分子量分别约为 ~133.9 & 59.1 kDa。

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100 μg   询价  

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描述

Carboxyester lipase/CEL protein plays a crucial role in cellular processes as it catalyzes the hydrolysis of a variety of substrates, including cholesterol esters, phospholipids, lysophospholipids, diacylglycerols and triacylglycerols, and lipids of hydroxy fatty acids acid ester. FAHF). Carboxyl Ester Lipase/CEL Protein, Mouse (sf9, His) is the recombinant mouse-derived Carboxyl Ester Lipase/CEL protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Carboxyl Ester Lipase/CEL Protein, Mouse (sf9, His) is 534 a.a., with molecular weight of ~133.9 & 59.1 kDa, respectively.
研究背景

羧基酯脂肪酶 (CEL) 是一种催化多种底物水解的酶，包括胆固醇酯、磷脂、溶血磷脂、二酰基甘油和三酰基甘油以及羟基脂肪酸的脂肪酸酯 (FAHFA)。CEL 优先选择酯键远离羧酸酯基团的 FAHFA，并且它比饱和的 FAHFA 更快地水解不饱和的 FAHFA。这种酶活性对于胃肠道中膳食脂质的完全消化至关重要，并促进脂溶性维生素的吸收。CEL 在脂质代谢中的作用强调了其在营养吸收的整个过程中的重要性，并强调了其在维持脂质稳态中的重要性。
种属

Mouse
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

Q64285 (M1-L534)
基因 ID

12613  [NCBI]
蛋白结构
N-term
CEL (M1-L534)
Accession # Q64285
His
C-term
同用名
Bile salt-activated lipase; BAL; BSSL; Cel; Lip1
分子量

Approximately 133.9&59.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Carboxyl Ester Lipase/CEL Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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