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  4. Carboxylesterase 1 蛋白, Human (549a.a, HEK293, His)

Carboxylesterase 1 蛋白, Human (549a.a, HEK293, His)

目录号: HY-P703886
COA 产品使用指南 技术支持

Carboxylesterase 1 蛋白, Human (549a.a, HEK293, His) 是重组人源的 Carboxylesterase 1 蛋白，由 HEK293 表达，带有 C-His 标签。

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规格 价格 是否有货 数量
10 μg ￥1000 In-stock
50 μg ￥2800 In-stock
100 μg ￥4500 In-stock
> 100 μg   询价  

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描述

Carboxylesterase 1 Protein, Human (549a.a, HEK293, His) is the recombinant human-derived Carboxylesterase 1 protein, expressed by HEK293, with C-His tag.
研究背景

Carboxylesterase 1 是一种参与外源性物质解毒和酯类及酰胺前药激活的酶。它能够水解芳香族和脂肪族酯类，但对酰胺或脂肪酰辅酶A 酯没有催化活性。该酶可将可卡因的甲酯基团水解为苯甲酰爱康宁，并催化可卡因的酯交换反应生成可卡乙烯。此外，Carboxylesterase 1 还表现出脂肪酸乙酯合成酶活性，催化油酸的乙酯化反应生成油酸乙酯，并将单酰基甘油水解为游离脂肪酸和甘油。它还能水解 2-花生四烯酸甘油和前列腺素，并将细胞中的胆固醇酯水解为游离胆固醇，促进逆向胆固醇转运（RCT）。具体而言，该酶首先促进巨噬细胞中游离胆固醇向细胞外胆固醇受体的流出，其次在肝脏中释放来自脂蛋白递送的胆固醇酯中的游离胆固醇，用于胆汁酸合成或直接分泌到胆汁中。by similar：该酶的功能还包括促进胆固醇代谢和药物代谢过程中的关键步骤。
生物活性

Measured by its ability to hydrolyze p-nitrophenylacetate.The specific activity is 18480 pmol/min/μg.

种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

P23141-1 (G18-L567)
基因 ID

1066
蛋白长度

Full Length of Isoform-1 Mature Protein
中文名
Carboxylesterase 1 蛋白
同用名
carrHuCarboxylesterase 1, His; CES1; Carboxylesterase 1; Cocaine Carboxylesterase; Serine Esterase 1; Triacylglycerol Hydrolase
预测分子量
62.1 kDa
分子量

Approximately 55.49 kDa,based on SDS-PAGE under reducing conditions.
糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (210 KB)

产品使用指南 (1213 KB)

Carboxylesterase 1 Protein, Human (549a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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