Carboxypeptidase A1/CPA1 蛋白, Mouse (HEK293, His)

Carboxypeptidase A1 (CPA1) is an enzyme responsible for catalyzing the release of a C-terminal amino acid; however, it exhibits limited or no activity with residues such as -Asp, -Glu, -Arg, -Lys, or -Pro. This specificity in substrate recognition underscores its role as a carboxypeptidase with selectivity towards certain amino acid sequences at the C-terminus of peptides. Carboxypeptidase A1/CPA1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Carboxypeptidase A1/CPA1 protein, expressed by HEK293 , with C-His labeled tag.

羧肽酶 A1 (CPA1) 是一种在催化肽 C 端氨基酸释放中发挥关键作用的酶。然而，对于具有 C 末端残基（例如 -Asp、-Glu、-Arg、-Lys 或 -Pro）的肽，其活性有限或不存在。这种底物特异性表明 CPA1 在其切割偏好方面表现出选择性，更喜欢肽 C 末端的某些氨基酸残基。该酶释放 C 末端氨基酸的作用有助于各种生理过程，例如消化系统中蛋白质的消化，凸显了其在调节肽和蛋白质代谢中的重要性。

Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 5420.437 pmol/min/µg, as measured under the described conditions. (Activation description: The proenzyme needs to be activated by Trypsin for an activated form.)

Mouse

HEK293

C-His

Q7TPZ8 (N17-Y419)

109697  [NCBI]

Full Length of Mature Protein

NENFVGHQVLRISATDEAQVQKVRELEELEHLKLDFWRDPARAGLPIDVRVPFPTIQSVKAFLEYHDISYEIMIEDVQSLLDEEKQQMSAFQARALSTDAFNYATYHTLDEIYEFMDLLVTEHPQLVSKIQIGSTFEGRPINVLKFSTGGTNRPAIWIDTGIHSREWVTQASGVWFAKKITKDYGQEPTLTAILDNMDIFLEIVTNPDGFVYTHKTNRMWRKTRSHTEGSLCVGVDPNRNWDAAFGMPGASSNPCSETYRGKFPNSEVEVKSIVDFVTSHGNIKAFISIHSYSQLLLYPYGYTSEPAPDKEELDQLAKSAVTALTSLHGTKFKYGSIIDTIYQASGSTIDWTYSQGIKYSFTFELRDTGLRGFLLPASQIIPTAEETWLALLTIMDHTVKHPY

Approximately 42-49 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5 or 25 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.