1. 重组蛋白
  2. Enzymes & Regulators
  3. Carboxypeptidase
  4. Carboxypeptidase A
  5. Carboxypeptidase A1
  6. Carboxypeptidase A1/CPA1 蛋白, Mouse (HEK293, His)

Carboxypeptidase A1/CPA1 蛋白, Mouse (HEK293, His)

目录号: HY-P72868
COA 产品使用指南

羧肽酶 A1 (CPA1) 是一种负责催化 C 端氨基酸释放的酶;然而,它对 -Asp、-Glu、-Arg、-Lys 或 -Pro 等残基表现出有限的活性或没有活性。这种底物识别的特异性强调了其作为羧肽酶的作用,对肽 C 末端的某些氨基酸序列具有选择性。Carboxypeptidase A1/CPA1 蛋白, Mouse (HEK293, His) 是重组的 羧肽酶 A1/CPA1 蛋白,由 HEK293 表达,带有 C-His 标签。Carboxypeptidase A1/CPA1 蛋白, Mouse (HEK293, His) 全长 403 个氨基酸,分子量约为 ~42-49 kDa。

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描述

Carboxypeptidase A1 (CPA1) is an enzyme responsible for catalyzing the release of a C-terminal amino acid; however, it exhibits limited or no activity with residues such as -Asp, -Glu, -Arg, -Lys, or -Pro. This specificity in substrate recognition underscores its role as a carboxypeptidase with selectivity towards certain amino acid sequences at the C-terminus of peptides. Carboxypeptidase A1/CPA1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Carboxypeptidase A1/CPA1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carboxypeptidase A1/CPA1 Protein, Mouse (HEK293, His) is 403 a.a., with molecular weight of ~42-49 kDa.

研究背景

羧肽酶 A1 (CPA1) 是一种在催化肽 C 端氨基酸释放中发挥关键作用的酶。然而,对于具有 C 末端残基(例如 -Asp、-Glu、-Arg、-Lys 或 -Pro)的肽,其活性有限或不存在。这种底物特异性表明 CPA1 在其切割偏好方面表现出选择性,更喜欢肽 C 末端的某些氨基酸残基。该酶释放 C 末端氨基酸的作用有助于各种生理过程,例如消化系统中蛋白质的消化,凸显了其在调节肽和蛋白质代谢中的重要性。

生物活性

Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 5420.437 pmol/min/µg, as measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q7TPZ8 (N17-Y419)

基因 ID

109697  [NCBI]

蛋白结构
N-term
CPA1 (N17-Y419)
Accession # Q7TPZ8
His
C-term
同用名
Carboxypeptidase A1; Cpa1
氨基酸序列

NENFVGHQVLRISATDEAQVQKVRELEELEHLKLDFWRDPARAGLPIDVRVPFPTIQSVKAFLEYHDISYEIMIEDVQSLLDEEKQQMSAFQARALSTDAFNYATYHTLDEIYEFMDLLVTEHPQLVSKIQIGSTFEGRPINVLKFSTGGTNRPAIWIDTGIHSREWVTQASGVWFAKKITKDYGQEPTLTAILDNMDIFLEIVTNPDGFVYTHKTNRMWRKTRSHTEGSLCVGVDPNRNWDAAFGMPGASSNPCSETYRGKFPNSEVEVKSIVDFVTSHGNIKAFISIHSYSQLLLYPYGYTSEPAPDKEELDQLAKSAVTALTSLHGTKFKYGSIIDTIYQASGSTIDWTYSQGIKYSFTFELRDTGLRGFLLPASQIIPTAEETWLALLTIMDHTVKHPY

分子量

Approximately 42-49 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5 or 25 mM Tris-HCL, 150 mM NaCl, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Carboxypeptidase A1/CPA1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Carboxypeptidase A1/CPA1 Protein, Mouse (HEK293, His)
目录号:
HY-P72868
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