2. 重组蛋白
  3. Enzymes & Regulators
  4. Carboxypeptidase
  5. Carboxypeptidase A
  6. CPA4
  7. Carboxypeptidase A4/CPA4 蛋白, Mouse (HEK293, His)

Carboxypeptidase A4/CPA4 蛋白, Mouse (HEK293, His)

目录号: HY-P70078
产品使用指南

羧肽酶 A4 (CPA4) 蛋白是一种与组蛋白高度乙酰化途径相关的金属蛋白酶，表明可能参与表观遗传调控。CPA4 对 C 端氨基酸（例如 -Phe、-Leu、-Ile、-Met、-Tyr 和 -Val）具有特异性，并且可以选择性切割底物，表明其在调节蛋白质组成和功能中发挥作用。Carboxypeptidase A4/CPA4 蛋白, Mouse (HEK293, His) 是重组的 羧肽酶 A4/CPA4 蛋白，由 HEK293 表达，带有 C-6*His 标签。Carboxypeptidase A4/CPA4 蛋白, Mouse (HEK293, His) 全长 404 个氨基酸，分子量约为 ~50.0 kDa。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

Carboxypeptidase A4 (CPA4) protein is a metalloprotease associated with the histone hyperacetylation pathway, suggesting a possible involvement in epigenetic regulation. CPA4 is specific for C-terminal amino acids (such as -Phe, -Leu, -Ile, -Met, -Tyr, and -Val) and can selectively cleave substrates, suggesting a role in regulating protein composition and function. Carboxypeptidase A4/CPA4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Carboxypeptidase A4/CPA4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Carboxypeptidase A4/CPA4 Protein, Mouse (HEK293, His) is 404 a.a., with molecular weight of ~50.0 kDa.
研究背景

羧肽酶 A4 (CPA4) 蛋白被归类为金属蛋白酶，与组蛋白高度乙酰化途径有关，表明在表观遗传调控中具有潜在作用。该酶在切割 C 端氨基酸方面具有特异性，优先切割 -Phe、-Leu、-Ile、-Met、-Tyr 和 -Val 等残基。CPA4 的底物选择性暗示其参与通过靶向切割调节蛋白质的组成和功能。CPA4 微妙的酶活性强调了其在与组蛋白修饰和蛋白质周转相关的细胞过程中的潜在重要性。
种属

Mouse
表达系统

HEK293
标签

C-6*His
蛋白编号

Q6P8K8 (G17-Y420)
基因 ID

71791  [NCBI]
蛋白结构
N-term
CPA4 (G17-Y420)
Accession # Q6P8K8
6*His
C-term
同用名
rMuCarboxypeptidase A4/CPA4, His; CPA4; Carboxypeptidase A4
氨基酸序列

GRDKFFGDQVFRINVRNGDEIRKLTELVNSDHLKLSVWKSPSTFDRPVDILVPSVSLLPVKSFLKSQGLDYSVTIEDLQALLDNEDEEMQHNEGIERSGDFNYGAYHPLEAIYHEMDSIATDFPELVSRVKIGETFEKRPMYVLKFSTGGGKKRPAIWLNAGIHAREWISQATAIWTARKIVTDYKKDPAITSILKKVDIFLLPVANPDGYVYTQSQNRLWRKTRSRNPGSRCVGADPNRNWNASFAGEGTSDNPCSEVYHGSHPNSEVEVKSVVDFIQKHGNFKCFIDLHSYSQLLMYPYGYTVKKAPDAEELDDVARNAAQALASLSGTKYRVGPTCTTVYPASGSSVDWAYDNGIKYAFTFELRDTGYYGFLLPASQIIPTAEETWLGLKTIMEHVRDHLY

分子量

Approximately 50.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Carboxypeptidase A4/CPA4 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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