Cardiac troponin I/Tnni3 蛋白, Mouse (His-SUMO)

Cardiac troponin I, also known as Tnni3, plays a key role as an inhibitory subunit in the troponin complex, a key regulator of calcium sensitivity in actomyosin ATPase activity in striated muscle. Tnni3 interacts with TRIM63, emphasizing its involvement in complex cellular processes. Cardiac troponin I/Tnni3 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Cardiac troponin I/Tnni3 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Cardiac troponin I/Tnni3 Protein, Mouse (His-SUMO) is 210 a.a., with molecular weight of ~45 kDa.

心肌肌钙蛋白 I，也称为 Tnni3，作为肌钙蛋白复合物中的抑制亚基发挥着关键作用，肌钙蛋白复合物是横纹肌肌动球蛋白 ATP 酶活性中钙敏感性的关键调节剂。该蛋白与 TRIM63 相互作用，凸显其参与复杂的细胞过程。此外，它还表现出与肌动蛋白和原肌球蛋白（肌肉收缩调节中不可或缺的成分）的结合亲和力。与 STK4/MST1 的相互作用进一步表明了一个关联网络，该网络有助于心肌肌钙蛋白 I 在协调控制肌肉生理学的复杂机制中发挥多方面的功能。

Mouse

E. coli

N-SUMO;N-6*His

P48787 (2A-211G)

21954  [NCBI]

ADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG

Approximately 45 kDa.The reducing (R) protein migrat es as 45 kDa in SDS-PAGE may be due to molecular structure of protein.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.