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  3. CARHSP1 蛋白, Human

CARHSP1 蛋白, Human

目录号: HY-P76766A
COA 产品使用指南

CARHSP1 蛋白通过与 mRNA 结合来关键调节转录后基因表达,影响其稳定性并影响 RNA 稳态。此外,CARHSP1 与单链 DNA 的体外结合证明了分子相互作用的多功能性。CARHSP1 蛋白, Human 是重组的 CARHSP1 蛋白,由 E. coli 表达,不带标签。CARHSP1 蛋白, Human 全长 146 个氨基酸,分子量为 18-23 kDa。

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描述

The CARHSP1 protein critically regulates post-transcriptional gene expression by binding to mRNA, affecting its stability and influencing RNA homeostasis. Furthermore, in vitro binding of CARHSP1 to single-stranded DNA demonstrates the versatility of the molecular interaction. CARHSP1 Protein, Human is the recombinant human-derived CARHSP1 protein, expressed by E. coli , with tag free. The total length of CARHSP1 Protein, Human is 146 a.a., with molecular weight of 18-23 kDa.

研究背景

CARHSP1 蛋白通过与 mRNA 结合并调节靶 mRNA 的稳定性,在转录后基因调控中发挥着至关重要的作用,突显了其参与与 RNA 稳态相关的细胞过程。此外,CARHSP1 表现出体外结合单链 DNA 的能力,表明其分子相互作用具有潜在的多功能性。在结构上,该蛋白质形成同型二聚体,表明其功能活性可能与其二聚体状态有关。此外,CARHSP1 与 STYX 相互作用,强调其与特定结合伙伴的关联以及可能参与更大的分子复合物或信号通路。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9Y2V2 (S2-S147)

基因 ID

23589

蛋白结构
N-term
CARHSP1 (S2-S147)
Accession # Q9Y2V2
C-term
同用名
Calcium-regulated heat-stable protein 1; Calcium-regulated heat-stable protein of 24 kDa; CRHSP-24
氨基酸序列

SSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

分子量

18-23 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CARHSP1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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