Caspase-1/CASP1 蛋白, Human (GST)

Caspase-1/CASP1 is a thiol protease complex involved in inflammation by catalyzing the cleavage of IL1B, IL18, and Gasdermin-D (GSDMD). It activates a pro-inflammatory response, releases mature cytokines IL1B and IL18, and initiates pyroptosis through cleavage of GSDMD. Caspase-1/CASP1 Protein, Human (GST) is the recombinant human-derived Caspase-1/CASP1 protein, expressed by E. coli , with N-GST labeled tag. The total length of Caspase-1/CASP1 Protein, Human (GST) is 150 a.a., with molecular weight of ~43.8 kDa.

Caspase-1/CASP1 亚基 p10 蛋白是一种硫醇蛋白酶，通过催化与炎症相关的前体蛋白的蛋白水解裂解，复杂地参与各种炎症过程。这包括白细胞介素 1 β (IL1B) 和白细胞介素 18 (IL18)、关键炎症细胞因子以及细胞焦亡诱导剂 Gasdermin-D (GSDMD)，将它们转化为活性成熟肽。作为细胞免疫的关键启动子，Caspase-1 在炎症小体复合物形成时激活促炎反应，导致成熟细胞因子 IL1B 和 IL18 的释放，这些细胞因子在多种炎症途径中发挥着关键作用。Caspase-1 的活性延伸至通过裂解 GSDMD 来启动焦亡，这是一种程序性裂解细胞死亡途径。值得注意的是，与白细胞介素的裂解不同，GSDMD 的识别和裂解依赖于 CASP1 上的外切位点界面，这强调了其多功能且复杂的调节作用。此外，Caspase-1 响应细菌感染而激活 CASP7，促进质膜修复，并通过在 DNA 病毒感染期间激活炎症小体时裂解 CGAS 来控制抗病毒免疫。在凋亡细胞中，Caspase-1 会裂解 SPHK2，后者在细胞外仍具有酶活性。尽管 Caspase-1 参与多种细胞过程，但它对细胞凋亡不活跃。

Specific Activity is 4080.135 units/mg. Unit definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Human

E. coli

N-GST

P29466 (N120-N269)

834  [NCBI]

NPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKN

Approximately 43.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.