1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Caspase
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  5. Caspase-7/CASP7 蛋白, Human (His)

Caspase-7/CASP7 蛋白, Human (His)

目录号: HY-P74347
COA 产品使用指南

Caspase-7/CASP7 蛋白是一种硫醇蛋白酶,参与程序性细胞死亡过程,例如细胞凋亡、细胞焦亡和粒酶介导的死亡。它通过启动半胱天冬酶(CASP8、CASP9 和/或 CASP10)激活并裂解 CLSPN、PARP1、PTGES3 和 YY1,介导细胞凋亡。Caspase-7/CASP7 蛋白, Human (His) 是重组的 Caspase-7/CASP7 蛋白,由 E. coli 表达,带有 C-His 标签。Caspase-7/CASP7 蛋白, Human (His) 全长 303 个氨基酸,分子量分别约为 ~20 & 11 kDa。

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规格 价格 是否有货 数量
10 μg ¥2150 In-stock
50 μg   询价  
100 μg   询价  

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描述

Caspase-7/CASP7 protein is a thiol protease involved in programmed cell death processes such as apoptosis, pyroptosis, and granzyme-mediated death. It is activated by initiating caspases (CASP8, CASP9 and/or CASP10) and cleaves CLSPN, PARP1, PTGES3 and YY1, mediating apoptosis. Caspase-7/CASP7 Protein, Human (His) is the recombinant human-derived Caspase-7/CASP7 protein, expressed by E. coli , with C-His labeled tag. The total length of Caspase-7/CASP7 Protein, Human (His) is 303 a.a., with molecular weight of ~20 & 11 kDa, respectively.

研究背景

Caspase-7/CASP7 蛋白是一种硫醇蛋白酶,复杂地参与各种程序性细胞死亡过程,包括细胞凋亡、细胞焦亡或颗粒酶介导的程序性细胞死亡。这种多面蛋白在被起始半胱天冬酶(CASP8、CASP9 和/或 CASP10)激活后催化靶蛋白(如 CLSPN、PARP1、PTGES3 和 YY1)的蛋白水解裂解,从而介导细胞凋亡的执行阶段。值得注意的是,CASP7 显示出对 Asp-Glu-Val-Asp (DEVD) 共有序列的偏好,并对替代非规范序列表现出可塑性。在细菌感染引起的炎症反应中,CASP7 通过裂解和激活细胞外环境中的鞘磷脂磷酸二酯酶 SMPD1,促进膜修复,发挥着至关重要的作用。此外,CASP7 调节肠上皮细胞的焦亡和肝细胞中颗粒酶介导的程序性细胞死亡,有助于膜修复并分别抵消 Gasdermin-D (GSDMD) 孔和穿孔素 (PRF1) 孔的作用。此外,CASP7 通过裂解抗病毒蛋白 CGAS、IRF3 和 MAVS,抑制病毒诱导的细胞凋亡过程中 I 型干扰素的产生。尽管具有多种作用,CASP7 本身缺乏酶活性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

P55210 (M1-Q303)

基因 ID

840  [NCBI]

蛋白结构
N-term
CASP7 (M1-Q303)
Accession # P55210
His
C-term
同用名
CMH-1; CASP-7; Caspase-7; MCH3; ICE-LAP3
分子量

Approximately 20&11 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES, 100 mM NaCl, 1 mM EDTA, 0.10% Sucrose, 0.1% chaps, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Caspase-7/CASP7 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Caspase-7/CASP7 Protein, Human (His)
目录号:
HY-P74347
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