Caspase-8/CASP8 subunit p18 蛋白, Human (His)

The Caspase-8/CASP8 subunit p18 protein is a key thiol protease that acts as a molecular switch in programmed cell death processes, including apoptosis, necroptosis, and pyroptosis. It is essential for preventing tissue damage by inducing exogenous apoptosis by cleaving and activating effector caspases. Caspase-8/CASP8 subunit p18 Protein, Human (His) is the recombinant human-derived Caspase-8/CASP8 subunit p18 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Caspase-8/CASP8 subunit p18 Protein, Human (His) is 158 a.a., with molecular weight of ~21.9 kDa.

Caspase-8/CASP8 亚基 p18 蛋白是一种关键的硫醇蛋白酶，作为分子开关协调程序性细胞死亡过程，例如细胞凋亡、坏死性凋亡和细胞焦亡。这种起始蛋白酶对于防止胚胎发育和成年期间的组织损伤至关重要，它通过裂解和激活效应半胱天冬酶（包括 CASP3、CASP4、CASP6、CASP7、CASP9 和 CASP10）来诱导外源性细胞凋亡。作为响应 TNFRSF6/FAS 或 TNFRSF1A 信号而形成的死亡诱导信号复合物 (DISC) 的引发剂，它释放活性二聚酶，从而激活下游凋亡蛋白酶。此外，Caspase-8 通过裂解 RIPK1 负向调节坏死性凋亡，并作为细胞焦亡的调节剂发挥作用，启动gasdermin-C 和 -D 的裂解和激活。这种多面蛋白酶通过裂解 TLR3 或 TLR4 下游的 N4BP1 并使 N4BP1 失活，从而促进细胞因子的产生，从而在先天免疫中发挥作用。虽然缺乏催化位点，Caspase-8 可能会干扰复合物的促凋亡活性。

Caspase-8/CASP8 subunit p18 Protein, Human (His) lacks of Caspase-8 enzyme activity because it contains only one subunit.

Human

E. coli

N-6*His

Q14790 (S217-D374)

841  [NCBI]

SESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETD

Approximately 21.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.