1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. Catalase 蛋白, Human

Catalase 蛋白, Human

目录号: HY-P7744
COA 产品使用指南

Catalase 蛋白, Human是在大肠杆菌中表达的一个约 60.0 kDa 的人重组过氧化氢酶蛋白。Catalase 是 H2O2 和活性氮代谢的关键酶,其在肿瘤中的表达和定位发生明显改变。

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规格 价格 是否有货 数量
10 μg ¥1654 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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MCE 顾客使用本产品发表的科研文献

  • 客户验证
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Catalase Protein, Human is an approximately 60.0 kDa human catalase protein expressed in E.coli. Catalase is a key enzyme in metabolism of H2O2 and reactive nitrogen species, and its expression and localization is markedly altered in tumors[1].

研究背景

过氧化氢酶是人体防御氧化应激的关键抗氧化酶。过氧化氢酶将活性氧过氧化氢转化为水和氧气,从而减轻过氧化氢的毒性作用[1][2]

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P04040 (A2-L527)

基因 ID

847  [NCBI]

蛋白结构
N-term
Catalase (A2-L527)
Accession # P04040
C-term
同用名
rHuCatalase; Catalase; CAT
氨基酸序列

ADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITKYSKAKVFEHIGKKTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDPILFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNANGEAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL

分子量

Approximately 60.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filter solution of PBS, PH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

Catalase Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Catalase Protein, Human
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