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  3. CBX5 蛋白, Human (His, Myc)

CBX5 蛋白, Human (His, Myc)

目录号: HY-P704132
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CBX5 蛋白, Human (His, Myc) 是重组人源的 CBX5 蛋白,由 E. coli 表达,带有 N-10*His & C-Myc 标签。

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描述

CBX5 Protein, Human (His, Myc) is the recombinant human-derived CBX5 protein, expressed by E. coli, with N-10*His & C-Myc tag.

研究背景

CBX5 是异染色质 (heterochromatin) 的组成成分,能够识别并结合组蛋白 H3 尾部甲基化的 'Lys-9' (H3K9me),从而引起表观遗传抑制 (epigenetic repression)。然而,当组蛋白 H3 的 'Tyr-41' 被磷酸化 (H3Y41ph) 时,它会被排除在染色质之外。CBX5 可能通过与 lamin-B 受体 (LBR) 的相互作用,促进异染色质与内核膜的关联。此外,它还通过与 MIS12 复合体亚基 NSL1 的相互作用参与动粒 (kinetochore) 的形成,并且是内着丝粒 (inner centromere) 形成所必需的。

种属

Human

表达系统

E. coli

标签

N-10*His;C-Myc

蛋白编号

P45973 (M1-S191)

基因 ID

23468

蛋白结构
N-term
10*His
CBX5 (M1-S191)
Accession # P45973
Myc
C-term
蛋白长度

Full Length

中文名
人色盒蛋白同源物 5
同用名
Antigen p25; CBX5; CBX5_HUMAN; CG8409; Chromobox 5; Chromobox homolog 5 (HP1 alpha homolog; Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Epididymis luminal protein 25; HEL25; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha; Su(var)205
氨基酸序列

MGKKTKRTADSSSSEDEEEYVVEKVLDRRVVKGQVEYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKYKKMKEGENNKPREKSESNKRKSNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS

预测分子量
29.7 kDa
分子量

Approximately 29.7 kDa,based on SDS-PAGE under reducing conditions.

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CBX5 Protein, Human (His, Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CBX5 Protein, Human (His, Myc)
目录号:
HY-P704132
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