CCL11 蛋白, Rat

CCL11 protein directly promotes eosinophil accumulation in allergic reactions, selectively affecting eosinophils without influencing other immune cells. It binds to CCR3, emphasizing its role in eosinophil-mediated processes. The interaction between CCL11 and its receptor provides insights into the regulation of allergic reactions. CCL11 Protein, Rat is the recombinant rat-derived CCL11 protein, expressed by E. coli , with tag free.

CCL11 蛋白响应过敏原的存在，在促进嗜酸性粒细胞积聚方面发挥直接作用，这是过敏性炎症反应的一个显着特征。值得注意的是，CCL11 选择性地影响嗜酸性粒细胞的募集，而对淋巴细胞、巨噬细胞或中性粒细胞没有类似的影响。这种特定的活性凸显了其在协调过敏性免疫反应中的重要性。CCL11 通过与 CCR3 结合来实现其作用，强调了这种趋化因子参与嗜酸性粒细胞介导的过程。CCL11 及其受体之间复杂的相互作用揭示了过敏反应期间控制免疫细胞选择性募集的分子机制，为炎症过程的调节提供了宝贵的见解。

1.Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/mL.
2.Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 6.052 ng/mL, corresponding to a specific activity is 1.65×10⁵ U/mg.

Rat

E. coli

Tag Free

P97545 (H24-P97)

29397  [NCBI]

Full Length of Mature Protein

HPGSIPTSCCFTMTSKKIPNTLLKSYKRITNNRCTLKAIVFKTKLGKEICADPKKKWVQDATKHLDQKLQTPKP

Approximately 10 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.