CCL11 蛋白, Rhesus macaque (N-His)

The CCL11 protein directly promotes eosinophil accumulation in response to allergens, a key feature of allergic inflammation, without significantly affecting lymphocytes, macrophages, or neutrophils. This selective recruitment highlights the specificity of CCL11 in the coordination of immune responses, particularly in allergy. CCL11 Protein, Rhesus macaque (N-His) is the recombinant Rhesus Macaque-derived CCL11 protein, expressed by E. coli , with N-6*His labeled tag.

针对过敏原的存在，CCL11蛋白直接促进嗜酸性粒细胞的积累，这是过敏性炎症反应的一个突出特征，而对淋巴细胞、巨噬细胞或中性粒细胞没有显着影响。这种选择性招募强调了 CCL11 在协调免疫反应方面的特异性，特别是在过敏反应的情况下。该蛋白通过与 CCR3 结合来实现这一效果，强调其与特定受体的结合，调节嗜酸性粒细胞响应过敏刺激的迁移和激活。

Measured in a cell proliferation assay using HUVEC cells.The ED₅₀ for this effect is 2.326 ng/mL, corresponding to a specific activity is 4.30×10⁵ units/mg.

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  Measured in a cell proliferation assay using HUVEC cells.The ED₅₀ for this effect is 2.326 ng/mL, corresponding to a specific activity is 4.30×10⁵ units/mg.

Rhesus Macaque

E. coli

N-6*His

Q8MIT7 (G24-P97)

574218  [NCBI]

Full Length of Mature Protein

GPDSVATTCCFTLTNKKIPLQRLESYRRIISGKCPQKAVIFKTKLAKDICADPKKKWVQDSMKYLDRKSPTPKP

Approximately 11 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.