CCL9/10/MIP-1 gamma 蛋白, Mouse

CCL9 Protein, Mouse, is often produced in macrophages, osteoclasts, and functions as a cell survival factor.

CCL9 是一种趋化因子，也称为巨噬细胞炎症蛋白-1 γ (MIP-1γ)，通常在巨噬细胞、破骨细胞中产生，并作为细胞生存因子发挥作用。在 CC 趋化因子家族成员中，小鼠 CCL9 与小鼠 CCL6 (C10)、CCL3 (MIP-1α) 和 CCL4 (MIP-1β) 分别具有 45%、24% 和 20% 的 aa 序列同一性。CCL9/CCR1 信号传导对于将骨髓祖细胞募集到肠道肿瘤中非常重要，从而导致侵袭增强。CCL9 在荷瘤小鼠转移前肺中或与肿瘤细胞共培养的未成熟骨髓细胞中高度诱导，在转移部位肿瘤细胞的存活中发挥着关键作用^{[1] [2]}。

1.The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/mL.
2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is ≤0.34 μg/mL, corresponding to a specific activity is ≥2.94×10³ U/mg.

• 
  Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.34 μg/mL, corresponding to a specific activity is 2.94×103 U/mg.

Mouse

E. coli

Tag Free

P51670 (Q22-Q122)

20308  [NCBI]

QITHATETKEVQSSLKAQQGLEIEMFHMGFQDSSDCCLSYNSRIQCSRFIGYFPTSGGCTRPGIIFISKRGFQVCANPSDRRVQRCIERLEQNSQPRTYKQ

Approximately 12-15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 300 mM NaCl, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hangyi H Yan, et al. CCL9 Induced by TGFβ Signaling in Myeloid Cells Enhances Tumor Cell Survival in the Premetastatic Organ. Cancer Res. 2015 Dec 15;75(24):5283-98.  [Content Brief]

  [2]. Xinyan Zhao, et al. CCL9 is secreted by the follicle-associated epithelium and recruits dome region Peyer's patch CD11b+ dendritic cells. J Immunol. 2003 Sep 15;171(6):2797-803.  [Content Brief]

  [3]. Baohua Li, et al. CCL9/CCR1 induces myeloid?derived suppressor cell recruitment to the spleen in a murine H22 orthotopic hepatoma model. Oncol Rep. 2019 Jan;41(1):608-618.  [Content Brief]