CCN2/CTGF 蛋白, Rhesus macaque (HEK293, His)

CCN2 is a mitogen secreted by vascular endothelial cells and a mechanosensor of mesenchymal cell differentiation that secretes stromal cell proteins. CCN2 can sense appropriate mechanical stress and downregulate its expression accordingly. CCN2 is a classic epithelial-to-mesenchymal transition (EMT) and fibrosis marker. CCN2/CTGF Protein, Rhesus macaque (HEK293, His) is the recombinant Rhesus Macaque-derived CCN2/CTGF protein, expressed by HEK293, with C-His labeled tag. The total length of CCN2/CTGF Protein, Rhesus macaque (HEK293, His) is 323 a.a., with molecular weight of 39-45 kDa.

细胞通讯网络因子 2 (CCN2) 是血管内皮细胞分泌的有丝分裂原，是富含半胱氨酸的分泌基质细胞蛋白。CCN2参与软骨细胞的增殖和分化、多种细胞类型的细胞粘附，以及在多种类型的机械应力（如拉应力、压应力和剪切应力下）下的软骨细胞、成骨细胞和成纤维细胞等间充质细胞中的表达。此外，CCN2 表达受各种机械感觉信号通路的激活调节，例如机械感觉离子通道、整合素-焦点粘附-肌动蛋白动力学、Rho GTPase 家族成员、Hippo-YAP 信号传导和 G 蛋白偶联受体。身体。CCN2 基因中的某些多态性与系统性硬化症的较高发病率相关。在气道纤维化哮喘领域，CCN2 和纤连蛋白 (FN) 是经典的上皮间质转化 (EMT) 和纤维化标志物。A549 细胞中 CCN2 和 FN 因 AREG 刺激而上调。AREG 刺激引起的 EGFR/JNK/AP-1 激活进一步介导人肺上皮细胞中 TGF-β 诱导的 EMT。CCN2 还与血小板衍生生长因子相关。Sunitinib (HY-10255A) 通过 TOLLIP（Toll 相互作用蛋白）介导的内体相关途径诱导自噬，导致心肌细胞凋亡和心功能障碍。这种适应不良的自噬选择性地降解心肌细胞存活介质 CCN2。因此，舒尼替尼诱导的体内心脏功能障碍可以通过腺相关病毒血清型 9 (AAV9) 特异性敲低 Ccn2 的能力来模拟。

Immobilized Rhesus macaque CTGF His at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody Human IgG1 with a linear range of 0.1-2 ng/mL.

Rhesus Macaque

HEK293

C-His

H9FQD5-1 (Q27-A349)

714520  [NCBI]

39-45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.