CCT2 蛋白, Human (His-SUMO)

The CCT2 protein is an important component of the chaperone-containing T complex (TRiC), a molecular chaperone that assists in protein folding through ATP hydrolysis. CCT2 Protein, Human (His-SUMO) is the recombinant human-derived CCT2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of CCT2 Protein, Human (His-SUMO) is 534 a.a., with molecular weight of ~66 kDa.

CCT2 作为含伴侣蛋白的 T 复合物 (TRiC) 的一个组成部分发挥作用，TRiC 是一种复杂的分子伴侣复合物，对于通过 ATP 水解进行蛋白质折叠至关重要。这种多功能复合物在介导包括 WRAP53/TCAB1 在内的各种蛋白质的折叠中发挥着至关重要的作用，从而影响端粒的维持。此外，作为 TRiC 复合物的一部分，CCT2 可能有助于 BBSome 的组装，BBSome 是纤毛发生所必需的复合物，可调节囊泡向纤毛的运输。TRiC 复合物参与肌动蛋白和微管蛋白的折叠，强调了其在细胞过程中的多方面功能（可能）。从结构上看，CCT2 是 TRiC 复合物的关键组成部分，TRiC 复合物是一种异源寡聚体组装体，其特征是两个堆叠的环，每个环的直径为 12 至 16 nm。除了其结构作用之外，CCT2 还与多种蛋白质相互作用，包括 PACRG、FLCN 和 DLEC1，进一步凸显了其在多种细胞过程中的重要性。

Human

E. coli

N-6*His;N-SUMO

P78371 (A2-C535)

10576  [NCBI]

ASLSLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLVDMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.