1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens Receptor Proteins
  3. Inhibitory checkpoint molecules Stimulatory immune checkpoint molecules NK Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Platelet CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Nectin-2/CD112
  6. Nectin-2/CD112 蛋白, Human (HEK293, His)

Nectin-2/CD112 蛋白, Human (HEK293, His)

目录号: HY-P7780
COA 产品使用指南

CD112 蛋白, Human (HEK293, His)是一种在 HEK 293 细胞中表达的,N 末端有 His 标签的重组人 CD112。CD112 蛋白 可用作人 DNAM-1 (CD226) 激活分子的细胞表面配体。

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规格 价格 是否有货 数量
10 μg ¥950 In-stock
50 μg ¥2850 In-stock
100 μg   询价  
250 μg   询价  

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描述

CD112 Protein, Human (HEK293, His) is a recombinant human CD112 expressed in HEK 293 cells with a His tag at the N-terminus. Recombinant Human CD112 can be used as cell surface ligands for the human DNAM-1 (CD226) activating molecule[1][2].

研究背景

Nectin-2 (CD112) is a 60 (Nectin-2 α) or 65 kDa (Nectin-2 δ) type I TM glycoprotein that expressed in a variety of cells. Nectin is a Ca2+-independent immunoglobulin-like intercellular adhesion molecule. Nectin comprises a family of at least four members nectin-1, nectin-2, nectin-3 and nectin-4. All the members have two or three splice variants (except nectin-4), and have an extracellular region containing three Ig-like domains, a single transmembrane region and a cytoplasmic region (except nectin-1γ). Nectin-1, nectin-2 and nectin-3 are ubiquitously expressed in a variety of cells. Nectin-2 and nectin-3 are also expressed in cells that lack cadherins. Human nectin-4 is expressed mainly in the placenta[1][2].

生物活性

Immobilized Human DNAM-1-Fc at 2μg/ml (100 μl/well) can bind recombinant Human CD112, His (HEK293-expressed) (rHuCD112, His). The ED50 of recombinant Human CD112, His (HEK293-expressed) (rHuCD112, His) is 0.3-1.5 ug/ml.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q92692-2 (Q32-L360)

基因 ID

5819  [NCBI]

中文名
重组人 CD112, His (HEK 293 表达)
同用名
rHuCD112, His; Poliovirus Receptor-Related Protein 2; Herpes Virus Entry Mediator B; Herpesvirus Entry Mediator B; CD112; PVRL2; HVEB; PRR2
氨基酸序列

QDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVSGTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDATLSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETPRASPRDVGPLHHHHHH

分子量

Approximately 50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Nectin-2/CD112 Protein, Human (HEK293, His)
目录号:
HY-P7780
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