1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Epithelial cell CD Proteins
  4. Nectin-3/CD113
  5. Nectin-3/CD113 蛋白, Human (309a.a, HEK293, His)

Nectin-3/CD113 蛋白, Human (309a.a, HEK293, His)

目录号: HY-P7782
COA 产品使用指南

CD113 蛋白, Human (HEK293, His)是一种在 HEK 293 细胞中表达的,N 末端有 His 标签的重组人 CD113。CD113 蛋白 是免疫球蛋白样细胞粘附分子中的一员,具有同质性和异质性细胞-细胞粘附活性。

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10 μg ¥950 In-stock
50 μg ¥2850 In-stock
100 μg   询价  

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描述

CD113 Protein, Human (HEK293, His) is a recombinant human CD113 expressed in HEK 293 cells with a His tag at the N-terminus. CD113 Protein is a member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities[1][2].

研究背景

Nectin 是一种不依赖 Ca2+ 的免疫球蛋白样细胞间粘附分子。Nectin 包含至少四个成员 nectin-1、nectin-2、nectin-3 和 nectin-4 的家族。所有成员均具有两个或三个剪接变体(nectin-4 除外),并具有包含三个 Ig 样结构域的胞外区、单个跨膜区和胞质区(nectin-1γ 除外)。Nectin-1、nectin-2 和 nectin-3 在多种细胞中普遍表达。Nectin-2 和 nectin-3 也在缺乏钙粘蛋白的细胞中表达。Nectin-4 主要在胎盘中表达。Nectin-3 具有三种剪接变体:nectin-3α(最大)、-3β(中)和-3γ(最小)。Nectin-3α 由三个胞外免疫球蛋白样结构域、一个跨膜片段和一个具有 C 端共有基序(用于结合 PDZ 结构域)的胞质区域组成[1][2]< /sup>。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9NQS3 (G58-P366)

基因 ID
蛋白结构
N-term
CD113 (G58-P366)
Accession # Q9NQS3
6*His
C-term
中文名
重组人 CD113, His (HEK 293 表达)
同用名
rHuCD113, His; Poliovirus Receptor-Related Protein 3; CDw113; Nectin-3; CD113; PVRL3; PRR3
氨基酸序列

GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPHHHHHH

分子量

Approximately 54 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, 5% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Nectin-3/CD113 Protein, Human (309a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Nectin-3/CD113 Protein, Human (309a.a, HEK293, His)
目录号:
HY-P7782
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