1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. B Cell CD Proteins NK Cell CD Proteins Stem Cell CD Proteins Receptor Tyrosine Kinases
  4. CD117/c-KIT
  5. CD117/c-Kit 蛋白, Mouse (HEK293, His)

CD117/c-Kit 蛋白, Mouse (HEK293, His)

目录号: HY-P72880
COA 产品使用指南

CD117/c-Kit 蛋白是一种酪氨酸蛋白激酶,作为 KITLG/SCF 的细胞表面受体,调节细胞存活、增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移、功能和黑素生成。CD117/c-Kit 蛋白, Mouse (HEK293, His) 是重组的 CD117/c-Kit 蛋白,由 HEK293 表达,带有 C-10*His 标签。CD117/c-Kit 蛋白, Mouse (HEK293, His) 全长 499 个氨基酸,分子量为 70-80 kDa。

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5 μg ¥450 In-stock
10 μg ¥750 In-stock
50 μg ¥2060 In-stock
100 μg ¥3500 In-stock
500 μg ¥10500 In-stock
1 mg ¥18000 In-stock
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描述

CD117/c-Kit protein is a tyrosine protein kinase that acts as a cell surface receptor for KITLG/SCF and regulates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, function, and melanogenesis . CD117/c-Kit Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD117/c-Kit protein, expressed by HEK293 , with C-10*His labeled tag. The total length of CD117/c-Kit Protein, Mouse (HEK293, His) is 499 a.a., with molecular weight of 70-80 kDa.

研究背景

CD117/c-Kit 蛋白是一种酪氨酸蛋白激酶,充当细胞因子 KITLG/SCF 的细胞表面受体。它在调节细胞存活和增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移、功能和黑素生成方面发挥着至关重要的作用。与 KITLG/SCF 结合后,CD117/c-Kit 激活多种信号通路,包括 PI3K/AKT、GRB2/RAS/RAF/MAPK 和 STAT。这导致各种下游效应子的磷酸化,例如 PIK3R1、PLCG1、SH2B2/APS、CBL 和转录因子 STAT1、STAT3、STAT5A 和 STAT5B。PLCG1 的激活导致产生重要的细胞信号分子二酰甘油和肌醇 1,4,5-三磷酸。CD117/c-Kit 信号传导受蛋白磷酸酶、快速内化和受体降解的调节。此外,它还促进 PTPN6/SHP-1、PTPRU 和其他蛋白质(如 CRK、LYN、SRC 和 SHC1)的磷酸化。

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD117/c-kit Fc Chimera is present at 0.5 μg/mL, can bind BiotinylatedRecombinant Mouse SCF/c-kit Ligand. The ED50 for this effect is 1.57 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD117/c-kit Fc Chimera is present at 0.5 μg/mL, can bind Biotinylated Recombinant Mouse SCF/c-kit Ligand. The ED50 for this effect is 1.57 ng/mL.
种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

P05532 (S25-T523)

基因 ID
蛋白结构
N-term
KIT (S25-T523)
Accession # P05532
10*His
C-term
中文名
重组鼠CD117蛋白
同用名
Mast/stem cell growth factor receptor Kit; SCFR; CD117; Kit; Sl
氨基酸序列

SQPSASPGEPSPPSIHPAQSELIVEAGDTLSLTCIDPDFVRWTFKTYFNEMVENKKNEWIQEKAEATRTGTYTCSNSNGLTSSIYVFVRDPAKLFLVGLPLFGKEDSDALVRCPLTDPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRCAAQRDGTWLHSDKFTLKVRAAIKAIPVVSVPETSHLLKKGDTFTVVCTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTISSARVDDSGVFMCYANNTFGSANVTTTLKVVEKGFINISPVKNTTVFVTDGENVDLVVEYEAYPKPEHQQWIYMNRTSANKGKDYVKSDNKSNIRYVNQLRLTRLKGTEGGTYTFLVSNSDASASVTFNVYVNTKPEILTYDRLINGMLQCVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNGTVECKASNDVGKSSAFFNFAFKGNNKEQIQAHT

分子量

70-80 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD117/c-Kit Protein, Mouse (HEK293, His)
目录号:
HY-P72880
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