1. 重组蛋白
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  3. Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins
  4. CD14
  5. CD14 蛋白, Human (HEK293, His)

CD14 蛋白, Human (HEK293, His)

目录号: HY-P7786
COA 产品使用指南

CD14 蛋白, Human (HEK293, His)是一种在 HEK 293 细胞中表达的,N 末端有 His 标签的重组人 CD14。CD14 蛋白 是传递 T 细胞表位的重组免疫球蛋白疫苗构建体的有效靶标。

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10 μg ¥950 In-stock
50 μg ¥2850 In-stock
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250 μg   询价  

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描述

CD14 Protein, Human (HEK293, His) is a recombinant human CD14 expressed in HEK 293 cells with a His tag at the N-terminus. CD14 Protein is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes[1][2].

研究背景

CD14, preferentially expressed on the surface of monocytes/macrophages, is a glycophosphatidylinositol‐linked protein, which is also part of the lipopolysaccharide (LPS) receptor complex. CD14 binds LPS and acts as a pattern recognition receptor (PRR). In addition to binding LPS and other bacterial products such as peptidoglycan and lipomannans, human CD14 has been suggested to mediate phagocytosis of bacteria and apoptotic cells[1][2].

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P08571 (T20-C352)

基因 ID

929  [NCBI]

中文名
重组人 CD14, His (HEK 293 表达)
同用名
rHuCD14, His; Monocyte Differentiation Antigen CD14; Myeloid Cell-Specific Leucine-Rich Glycoprotein; CD14
氨基酸序列

TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPAC

分子量

Approximately 54 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD14 Protein, Human (HEK293, His)
目录号:
HY-P7786
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