CD151 Protein-VLP, Human (HEK293)

CD151 Protein-VLP, Human (HEK293) is the recombinant human-derived CD151 protein, expressed by HEK293, with tag free tag.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

CD151 是 tetraspanin-enriched microdomains (TERMs) 的结构组分，作为受体聚集和信号传导的平台。通过与 integrin 和 non-integrin 蛋白的相互作用，CD151 参与多种细胞和分子机制，包括细胞间通讯、伤口愈合、血小板聚集、运输、细胞运动和血管生成。通过与 JAM-A/F11R 和 integrin ITGA3:ITGB1 的相互作用，CD151 促进 RAC1、CDC42 和 RhoGTPases 等信号分子的募集，从而促进上皮细胞的极化和肌动蛋白细胞骨架的重组，这是细胞迁移过程中的关键步骤。CD151 还调节 ITGA3:ITGB1 的糖基化模式以调控其活性，并在维持 central laminin-binding integrin ITGA6:ITGB4 粘附复合物的稳定性中起关键作用。此外，CD151 对肾脏中肾小球和肾小管基底膜的正确组装至关重要，并通过调节 integrin 信号传导促进 T 细胞活化，激活下游靶标 PTK2 和 MAPK1/MAPK3。在微生物感染方面，CD151 参与 human papillomavirus 16/HPV-16 的内吞作用，并促进 human cytomegalovirus 进入宿主细胞，影响其受体结合、膜融合或衣壳释放。

Human

HEK293

Tag Free

P48509 (G2-Y253)

977

Full Length

GEFNEKKTTCGTVCLKYLLFTYNCCFWLAGLAVMAVGIWTLALKSDYISLLASGTYLATAYILVVAGTVVMVTGVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYAYYQQLNTELKENLKDTMTKRYHQPGHEAVTSAVDQLQQEFHCCGSNNSQDWRDSEWIRSQEAGGRVVPDSCCKTVVALCGQRDHASNIYKVEGGCITKLETFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRSLKLEHY

溶液.

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.