2. 重组蛋白
  3. CD Antigens Enzymes & Regulators
  4. Endothelial cell CD Proteins Hydrolases (EC 3)
  5. BST1/CD157
  6. CD157 蛋白, Rat (HEK293, His)

CD157 蛋白, Rat (HEK293, His)

目录号: HY-P76205
产品使用指南

CD157蛋白催化NAD(+)合成cADPR并将cADPR水解为ADPR。 cADPR 充当第二信使，从细胞内储存中释放钙。 CD157 蛋白也可能有助于前 B 细胞的生长。CD157 蛋白, Rat (HEK293, His) 是重组的 CD157 蛋白，由 HEK293 表达，带有 C-His 标签。CD157 蛋白, Rat (HEK293, His) 全长 293 个氨基酸，分子量为 38-42 KDa。

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描述

CD157 protein catalyzes the synthesis of cADPR from NAD(+) and hydrolyzes cADPR to ADPR. cADPR acts as a second messenger, releasing calcium from intracellular stores. CD157 protein may also contribute to pre-B cell growth. CD157 Protein, Rat (HEK293, His) is the recombinant rat-derived CD157 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD157 Protein, Rat (HEK293, His) is 293 a.a., with molecular weight of 38-42 KDa.
研究背景

CD157 蛋白作为一种酶，催化 NAD(+) 合成环状 ADP-β-D-核糖 (cADPR)，并将 cADPR 水解为 ADP-D-核糖 (ADPR)。环状 ADPR 作为天然第二信使，触发细胞内储存的钙的释放，从而控制细胞内钙的动员。此外，CD157 蛋白可能在前 B 细胞的生长中发挥作用。
种属

Rat
表达系统

HEK293
标签

C-His
蛋白编号

Q63072 (M1-E293)
基因 ID

81506  [NCBI]
蛋白结构
N-term
CD157 (M1-E293)
Accession # Q63072
His
C-term
同用名
ADP-ribosyl cyclase; BST1; Cyclic ADP-ribose hydrolase 2; CD157
分子量

38-42 kDa.
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CD157 Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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