1. 重组蛋白
  2. CAR-T related Proteins CD Antigens
  3. B Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins
  4. CD20
  5. CD20/MS4A1 蛋白, Cynomolgus (85a.a, HEK293, His)

CD20/MS4A1 蛋白, Cynomolgus (85a.a, HEK293, His)

目录号: HY-P75433
COA 产品使用指南

CD20/MS4A1蛋白是一种B淋巴细胞膜蛋白,在细胞钙内流中起着至关重要的调节作用,这对于B淋巴细胞的发育、分化和激活至关重要。作为钙库操纵 (SOC) 通道的一部分,它在 B 细胞受体/BCR 激活时促进钙流入。CD20/MS4A1 蛋白, Cynomolgus (85a.a, HEK293, His) 是重组的 CD20/MS4A1 蛋白,由 HEK293 表达,带有 C-His 标签。CD20/MS4A1 蛋白, Cynomolgus (85a.a, HEK293, His) 全长 85 个氨基酸,分子量分别为 22.66 & 24.28 kDa。

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描述

CD20/MS4A1 protein is a B lymphocyte membrane protein that plays a crucial regulatory role in cellular calcium influx, which is essential for the development, differentiation and activation of B lymphocytes. As part of a store-operated calcium (SOC) channel, it promotes calcium influx upon B cell receptor/BCR activation. CD20/MS4A1 Protein, Cynomolgus (85a.a, HEK293, His) is the recombinant cynomolgus-derived CD20/MS4A1 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD20/MS4A1 Protein, Cynomolgus (85a.a, HEK293, His) is 85 a.a., with molecular weight of 22.66 & 24.28 kDa, respectively.

研究背景

CD20/MS4A1 蛋白是一种 B 淋巴细胞特异性膜蛋白,在调节细胞钙内流方面发挥着至关重要的作用,而钙内流对于 B 淋巴细胞的发育、分化和激活至关重要。它作为钙库操纵 (SOC) 通道的组成部分发挥作用,在 B 细胞受体/BCR 激活后促进钙流入。CD20/MS4A1 形成同源四聚体,有助于其结构组织和在钙信号传导中的功能作用。值得注意的是,它与细胞表面 IgM 的重链和轻链(BCR 的抗原结合成分)相互作用,突出了它在 B 细胞受体复合物中的参与,并强调了它在 B 细胞激活和免疫反应中的重要性。

生物活性

Measured in a cytotoxicity assay using Ramos cells. The ED50 this effect is 23.57 ng/mL, corresponding to a specific activity is 4.243×104 units/mg.

  • Measured in a cytotoxicity assay using Ramos cells. The ED50 for this effect is 23.57 ng/mL, corresponding to a specific activity is 4.243×104 units/mg.
种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

NP_001274241 (E213-P297)

基因 ID
蛋白结构
N-term
CD20 (E213-P297)
Accession # NP_001274241
His
C-term
同用名
Ms4a1; Cd20; Ly-44; Ms4a2; B-lymphocyte antigen CD20; B-cell differentiation antigen Ly-44; Lymphocyte antigen 44; CD antigen
氨基酸序列

ENEWRRTCSRPKSSVVLLSAEEKKEQVIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

分子量

22.66 & 24.28 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD20/MS4A1 Protein, Cynomolgus (85a.a, HEK293, His)
目录号:
HY-P75433
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