CD28 蛋白, Human/Cynomolgus (Biotinylated, HEK293, His-Avi)

The CD28 protein is critical for T cell activation, enhancing proliferation, cytokine production, and survival. When linked to TCR/CD3 and CD40L, CD28 promotes the production of IL4 and IL10, thereby regulating immune responses. CD28 Protein, Human/Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus, human-derived CD28 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of CD28 Protein, Human/Cynomolgus (Biotinylated, HEK293, His-Avi) is 134 a.a., with molecular weight of 38-50 kDa.

CD28 蛋白在 T 细胞激活、促进细胞增殖、细胞因子产生和 T 细胞存活中发挥着关键作用。与 TCR/CD3 和 CD40L 共刺激连接后，CD28 增强 T 细胞中 IL4 和 IL10 的产生，有助于免疫反应调节。此外，CD28 的异构体 3 促进 T 细胞中 CD40L 介导的 NF-κ-B 以及激酶 MAPK8 和 PAK2 的激活。该蛋白通过二硫键形成同型二聚体，并与多种分子相互作用，包括 DUSP14、CD80/B7-1、CD86/B7-2/B70 和 GRB2。同工型 3 与 CD40LG 特异性相互作用，突出了其在介导免疫反应和细胞信号传导途径中的多方面作用。

1. Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD28 at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CD28 Antibody hFc with the EC₅₀ ≤ 20.2 ng/mL determined by ELISA.
2. Immobilized Anti-CD28 Antibody, hFc Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC₅₀ ≤ 24.4 ng/mL determined by ELISA.

Cynomolgus; Human

HEK293

N-His;N-Avi

P10747 (N19-P152)

940  [NCBI]

38-50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.