CD31/PECAM-1 蛋白, Human (HEK293, His)

CD31/PECAM-1 Protein is a cell-cell adhesion protein that is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions^[1][2]. CD31/PECAM-1 Protein, Human (HEK293, His) is the recombinant human-derived CD31/PECAM-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD31/PECAM-1 Protein, Human (HEK293, His) is 574 a.a., with molecular weight of 75-110 kDa.

CD31/PECAM-1 蛋白在细胞间连接处的内皮细胞和 T 细胞亚群上大量表达，在血小板和大多数其他白细胞（如单核细胞和中性粒细胞）上表达较少^[1][2]。CD31/PECAM-1 蛋白同型地与其自身结合，也异型地与白细胞整合素 α v beta 3 结合^[3]。CD31/PECAM-1 蛋白是白细胞通过血管内皮细胞的细胞间连接进行跨内皮迁移所必需的^[4]。

Human

HEK293

C-6*His

AAH51822.1 (Q28-K601)

5175  [NCBI]

QENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKK

75-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 1 mM EDTA, pH 7.4 .

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.