CD31/PECAM-1 蛋白, Human

CD31/PECAM-1 Protein, a pivotal cell adhesion molecule, is essential for leukocyte transendothelial migration (TEM) in most inflammatory conditions. PECAM1 modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Signaling through PECAM-1 leads to the activation of neutrophils, monocytes and leukocytes. CD31/PECAM-1 Protein, Human is the recombinant human-derived CD31/PECAM-1 protein, expressed by HEK293 , with tag free. The total length of CD31/PECAM-1 Protein, Human is 574 a.a., with molecular weight of ~115 kDa.

CD31/PECAM-1 是一种关键的细胞粘附分子，对于大多数炎症条件下的白细胞跨内皮迁移 (TEM) 至关重要。Tyr-679 在 TEM 中的关键作用强调了它需要 PECAM-1 往返于侧缘回收区室 (LBRC) 的有效运输，这对于靶向迁移白细胞周围的 LBRC 膜至关重要。跨亲同性相互作用可能通过细胞连接促进内皮细胞与细胞的粘附。PECAM-1 的同种连接具有双重目的，通过传递分离信号来防止巨噬细胞介导的邻近活白细胞的吞噬作用，同时通过将凋亡白细胞束缚到吞噬细胞来促进巨噬细胞介导的对凋亡白细胞的吞噬作用。值得注意的是，PECAM-1 调节缓激肽受体 BDKRB2 激活，并调节内皮细胞中缓激肽和高渗休克诱导的 ERK1/2 激活。它与各种伙伴 (包括 BDKRB2、GNAQ、PTPN11、FER 和 CD177) 的相互作用进一步阐明了控制其多种细胞功能的复杂分子机制，而其反式同二聚化对于有效的细胞间相互作用至关重要^{[1][2] [3][4][5]}。

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5×10⁴ cells/well are added to rhCD31 coated plates (3.3 μg/mL,100 μL/well) in the presence of 20 ng/mL PMA, approximately 80.4% will adhere after 30 minutes at 37°C.

Human

HEK293

Tag Free

EAW94208.1 (Q28-K601)

5175  [NCBI]

QENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKK

Approximately 115 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 10 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.